脑室出血大鼠不同部位脑组织热休克蛋白70表达的差异

目的 观察实验性大鼠脑室出血后，不同部位脑组织热休克蛋白70（heat shock protein 70，HSP70）表达的差异。方法 18只成熟雄性SD大鼠随机均分为正常组、对照组和模型组，采用侧脑室注血法制备大鼠脑室出血动物模型，然后用酶联免疫吸附方法制作热休克蛋白70组织切片，在显微镜下直接计数阳性细胞；采用干湿重法计算脑含水量。结果 模型组大鼠脑组织HSP70阳性细胞数和含水量明显高于正常组和对照组（P分别为0.015和0.013），而且HSP70阳性细胞主要分布于皮层和皮层下，室周也有表达。结论 大鼠脑室内出血可引起远隔部位的皮层和皮层下组织HSP70的高度表达。

Expression Differences of Heat Shock Protein 70 in Brain tissues in Rat Model of Intraventricular Hemorrhage

Objective To observe the expression differences of Heat-shock Protein 70 （HSP70） in brain tissues in rat model of intraventricular hemorrhage （IVH）. Methods Eighteen matured-male SD rats were divided equally into normal, control and model group. The rat model of IVH, prepared by blood injecting into lateral cerebral ventricle directly, The tissue slice of HSPT0 was made by enzyme linked immunosorbent assay. The HSP70 positive cells were calculated by microscope directly. Water content of brain tissue was calculated by dry- wet weight method. Results The HSP70 positive cells and water content of brain tissue in model group were more than that of the normal or control groups（26.4 vs 2.4, 3.2; 55.33 vs 45.26, 48.38; P〈0.05 and P〈0.01, respectively）, and the expression of liSP70 appeared in cortex and subcortex areas mainly, also appeared in periventricular tissues. Conclusion IVH of rat model can induce a high