表皮生长因子受体信号通路在人子宫内膜癌移植瘤孕激素耐药机制中的作用

目的建立人子宫内膜癌皮下移植瘤孕激素耐药模型,探讨表皮生长因子受体(EGFR)下游信号通路在子宫内膜癌孕激素耐药机制中的作用。方法分别利用人子宫内膜癌孕激素敏感型Ishikawa细胞株和孕激素不敏感型Ishikawa-pLWERNL细胞株制备裸鼠皮下移植瘤模型(每组18只);成瘤后,分别将接种Ishikawa细胞株和接种Ishikawa-pLWERNL细胞株的裸鼠各自分成3组(每组6只),分别给予甲羟孕酮(MPA组)、吉非替尼(吉非替尼组)、生理盐水(对照组)。疗程结束后称取各组肿瘤湿重,采用Western blotting法检测肿瘤组织中EGFR、孕激素受体B(PR-B)、ERK1/2、p-ERK1/2、AKT及p-AKT蛋白的表达。结果在接种Ishikawa细胞株的裸鼠中,MPA组、吉非替尼组移植瘤湿重与对照组相比,分别减轻43.0%和31.5%,差异有统计学意义(P均<0.05)。在接种Ishikawa-pLWERNL细胞株的裸鼠中,吉非替尼组移植瘤湿重与对照组相比,减轻35.7%,差异有统计学意义(P<0.05);而MPA组移植瘤湿重与对照组相比,减轻2.9%,差异无统计学意义(P>0.05)。接种I...

Role of epidermal growth factor receptor signaling pathway in progestin resistance of human endometrial carcinoma xenografts

Objective To establish progestin-resistant models of human endometrial carcinoma xenografts,and explore the role of epidermal growth factor receptor(EGFR) signaling pathway in progestin resistance of human endometrial carcinoma xenografts.Methods Thirty-six nude mice were subcutaneously injected with human endometrial carcinoma progestin-sensitive Ishikawa cells(n=18) or progestin-insensitive Ishikawa-pLWERNL cells(n=18),and human endometrial carcinoma xenografts were formed.Then,both nude mice treated with...