| 抗人IgGFc和Fab单克隆抗体的鉴定及应用 |
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| 引用本文： | 沈心亮,陈红,魏庆兰,袁皓加,辛芳,刘新铭.抗人IgGFc和Fab单克隆抗体的鉴定及应用[J].细胞与分子免疫学杂志,1994(1). |
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| 作者姓名： | 沈心亮 陈红 魏庆兰 袁皓加 辛芳 刘新铭 |
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| 作者单位： | 卫生部兰州生物制品研究所 |
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| 摘 要： | 本文将采用木瓜蛋白酶水解和ＳＰＡ－Ｓｅｐｈａｒｏｓｅ４Ｂ柱亲和层析等手段获得的人ＩｇＧＦｃ和Ｆａｂ，以抗人ＩｇＧＦｃ和抗人ＩｇＧＦａｂ单抗为参比品（Ｓｉｇｍａ）．鉴定了细胞库中抗人ＩｇＧ系列的部分细胞林，得到分泌特异性抗人ＩｇＧＦｅ和抗人ＩｇＧＦａｂ单抗的细胞各一株。在此基础上。应用抗人ＩｇＧＦｃ及抗人ＩｇＧＦａｂ单抗分别制备了Ｓｅｐｈａｒｏｓｅ４Ｂ亲和层析柱，纯化了酶解的人ＩｇＧＦｃ和Ｆａｂ。经ＥＬＩＳＡ法鉴定，相互间无交叉反应。同时用此方法还制备了人抗ＨＢｅＦａｂ，并将此Ｆａｂ标记过氧化物酶。配制ＨＢｅＥＬＩＳＡ诊断盒，证明其生物学活性未受影响，而且消除了类风湿因子引起的ＨＢｅＡｇ假阳性现象。
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| 关 键 词： | 人IgGFc和Fab 抗体，单克隆 亲和层析 |
Identification and Application of Monoclonal Antibodies against Human IgG Fc and Fab |
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| Shen Xinliang,Chen Hong,Wei Qinglan,Yuan Haojia,Xin Fang,Liu Xinming.Identification and Application of Monoclonal Antibodies against Human IgG Fc and Fab[J].Journal of Cellular and Molecular Immunology,1994(1). |
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| Authors: | Shen Xinliang Chen Hong Wei Qinglan Yuan Haojia Xin Fang Liu Xinming |
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| Abstract: | After hydrolyzed with papain and separated on SPA affinity chromatography column,human IgG Fc and Fab were available.With these as coating antigens and Sigma monoclonal antibodiee to human IgG Fo and Fab as reference substances, antihnman IgG McAbs were identified and two of them were specific for human IgG Fc and Fab, respectively.On Sepharos e 4B affinity chromatography columns prepared with each of the two McAbs,human IgG Fc and Fab were purified and immunoassayed to be pure by ELISA.HBe ELISA kit was produced with above-mentioned purified human anti-HBe Fab labed ed by peroxidas e and demonstrated no effect on it's biological activities.This method has eliminated HBeAg pseudopositive phenomenon caused by rheumatoid factor. |
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| Keywords: | human IgG Fc and Fab antibody monoclenal affinity chromatography |
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