| 姜黄素抑制宫颈癌细胞株增殖和诱导凋亡的体外研究 |
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| 引用本文： | 夏娜,吴绪峰. 姜黄素抑制宫颈癌细胞株增殖和诱导凋亡的体外研究[J]. 数理医药学杂志, 2010, 23(1): 22-24 |
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| 作者姓名： | 夏娜 吴绪峰 |
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| 作者单位： | 1. 郧阳医学院附属人民医院老年病科
2. 武汉大学中南医院妇瘤科,武汉,430071 |
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| 摘 要： | 目的：探讨中药姜黄素（Cur）对子宫颈癌SiHa细胞株的增殖抑制和诱导凋亡的作用。方法：以不同浓度的姜黄素（10umol／L、20umol／L、30umol／L），分12h、24h、48h、72h4个时间点处理SiHa细胞，光镜观察细胞形态变化；用四甲基偶氮唑蓝（MTT）法测定细胞增殖抑制率；用透射电子显微镜及琼脂糖凝胶电泳检测凋亡的发生。结果：姜黄素可抑制SiHa细胞增殖，作用呈明显的时效和量效关系，差异有非常显著性（P〈0.01）；姜黄素可诱导SiHa细胞凋亡，透射电子显微镜下可见凋亡细胞；琼脂糖凝胶电泳出现细胞凋亡典型的DNA“梯状”条带。结论：姜黄素体外对SiHa细胞具有增殖抑制作用和促进凋亡作用。
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| 关 键 词： | 姜黄素 SiHa细胞 凋亡 琼脂糖凝胶电泳 |
The Study of Cervical Cancer SiHa cell Inhibition and Apoptosis Induced by Curcume |
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| Xia Na,et al. The Study of Cervical Cancer SiHa cell Inhibition and Apoptosis Induced by Curcume[J]. Journal of Mathematical Medicine, 2010, 23(1): 22-24 |
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| Authors: | Xia Na et al |
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| Affiliation: | Xia Na,et al(Gynecologic Oncology Department of Zhongnan Hospital,Wuhan University,Wuhan 430071) |
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| Abstract: | Objective:To investigate the SiHa cells growth inhibition and Apoptosis induced by Curcumin. Methods:SiHa cells were treated with various concentrations (10 umol/L,20 umol/L,30 umol/L ) of Curcume for different periods(12h,24h,48h,72h). Cell changing was observed by microscope;cell growth was measured by MTT. Apoptosis was detected by different methods including electron microscope and DNA gel electrophoresis. Results: After treated with different concentrations of Curcumin,the cell growth of SiHa was suppr... |
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| Keywords: | curcum in SiHa cell apoptosis DNA gel electrophoresis |
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