HPLC法同时测定鞘蕊苏中三种劳丹烷型二萜类成分

目的：建立HPLC法测定鞘蕊苏药材中异佛司可林、1-乙酰基佛司可林、1，6-二乙酰基佛司可林的含量。方法：采用luna C8柱（250mm×4．6mm），以乙腈-水梯度洗脱，流速为1．0mL／min，紫外检测波长为210nm，进样量20μL，采集时间40min。结果：异佛司可林、1-乙酰基佛司可林、1，6-二乙酰基佛司可林的线性范围分别为1．0～16．0μg（r=0．9998），0．9～7．2μg（r=0．9999），7．5～60μg（r=0．9998）．平均回收率（n=9）分别为97．8％，95．1％，97．6％．结论：本方法简便、准确，可为评价不同产地的鞘蕊苏药材中异佛司可林、1-乙酰基佛司可林、1，6-二乙酰基佛司可林的含量提供依据。

Simultaneous HPLC Determination of 3 Labdane Diterpenoids in Coleus forskohlin (wild)Briq.

Objective： To establish a HPLC method for determination of three constituents： including isoforskolin, 1 -actylforskolin and 1, 6 -diactylforskolin in Coleus forskohlin （wild） Briq. Methods： The reversed phase HPLC system consisted of a lunaCs column （250mm ×4. 6mm, 5μm）, The mobile phase consisted of acetonitrile （A） and H20 （B）. At a flow rate of 1. 0 mL/min, the gradient elutions were 50A： 50B to 35A ：65B in 30 min, 35A： 65B to 50A： 50B in next 10 min, 50A： 50B to 50A： 50B in last 5 min. The detection wavelength was 210 nm, the injection volume was 20μL and the column temperature was maintained at 25℃. Resuits： The Labdane Diterpenoids were successfully separated. The linear ranges of the Labdane Diterpenoids were 1.0 - 16.01μg （r =0. 9998）, 0. 9 -7.2μg （r =0. 9999）, 7.5 -60μg （r =0. 9998） respectively; The average recoveries of Labdane Diterpenoids were 97.8%, 85.1%, 97.6% respectively. Conclusion： The contents of the Labdane Diterpenoids in root of Coleus forskohlin （wild） Briq collected from different regions were determined. This method is rapid and accurate.