True microbiota involved in chronic lung infection of cystic fibrosis patients found by culturing and 16S rRNA gene analysis |
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| Authors: | Rudkjøbing Vibeke B Thomsen Trine R Alhede Morten Kragh Kasper N Nielsen Per H Johansen Ulla R Givskov Michael Høiby Niels Bjarnsholt Thomas |
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| Affiliation: | Department of Biotechnology, Chemistry, and Environmental Engineering, Faculty of Engineering and Science, Aalborg University, Aalborg, Denmark. |
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| Abstract: | Patients suffering from cystic fibrosis (CF) develop chronic lung infection. In this study, we investigated the microorganisms present in transplanted CF lungs (n = 5) by standard culturing and 16S rRNA gene analysis. A correspondence between culturing and the molecular methods was observed. In conclusion, standard culturing seems reliable for the identification of the dominating pathogens. |
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