| 兔视网膜色素上皮细胞的体外标记 |
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| 引用本文： | 喻晓兵,陈君,吴景天. 兔视网膜色素上皮细胞的体外标记[J]. 眼科研究, 2000, 18(5): 421-422 |
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| 作者姓名： | 喻晓兵 陈君 吴景天 |
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| 作者单位： | 1. 卫生部北京医院眼科,北京,100730
2. 沈阳中国医科大学第一临床学院眼科 |
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| 摘 要： | 目的 建立兔视网膜色素上皮（ＲＰＥ）细胞的体外标记方法。方法 兔ＲＰＥ细胞经传代培养并鉴定后，掺入溴脱氧嘧啶（ＢｒｄＵ）对细胞进行体外标记，通过免疫组织化学方法来显示标记的细胞。结果 ＢｒｄＵ标记后，所有的细胞核呈阳性染色。结论 提供一种简便易行的ＲＰＥ细胞标记方法，提示ＢｒｄＵ掺入法适用于所有培养细胞的体外标记。
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| 关 键 词： | 视网膜色素上皮细胞 溴脱氧嘧啶 免疫组织化学 |
| 修稿时间： | 1999-10-18 |
In vitro labelling of rabbit retinal pigment epithelial cells |
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| Yu Xiaobing,Chen Jun,Wu jingtian. In vitro labelling of rabbit retinal pigment epithelial cells[J]. Chinese Ophthalmic Research, 2000, 18(5): 421-422 |
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| Authors: | Yu Xiaobing Chen Jun Wu jingtian |
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| Abstract: | ObjectiveTo esta blish a labelling method for rabbit RPE cells in vitro.MethodsRabbit RPE cells were cultured and passaged in vitr o. RPE cells were labelled by BrdU incorporation,identified as RP by imm unocytochemistry methods.ResultsAll the nuclei of the RPE cells showed positive labelling with BrdU.ConclusionThis s tudy provides an easy way for labelling RPE cells,and suggests that cultu red cells could be labelled by BrdU inco rporation. |
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| Keywords: | retinal pigment epi thelium bromodeoxyuridine immunochemistry |
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