姜黄素抑制大鼠肝纤维化的研究

目的：探讨姜黄素抑制肝纤维化的效应及其分子机制。方法：培养大鼠肝星状细胞(HSC)并以刀豆蛋白(ConA)激活，再用不同剂量姜黄素处理；建立大鼠四氯化碳(CCl4)肝纤维化模型，以不同剂量姜黄素处理各模型动物，检测其血清透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)和Ⅵ型胶原(Ⅵ.C)水平，检测肝组织纤维化程度和肝组织及HSC基质金属蛋白酶-2(MMP-2)的表达。结果：CCl4成功诱导肝纤维化大鼠模型。与模型组比较，姜黄素治疗后能降低血清肝纤维化各项指标(HA,LN,PCⅢ及Ⅵ.C)的表达（P<0.05）,肝组织纤维化评分明显降低（P<0.05）。姜黄素处理后，纤维化大鼠肝组织MMP-2呈剂量依赖性减少（P<0.05），活化肝星状细胞MMP-2的表达呈剂量依赖性减少（P<0.05）。结论：姜黄素可通过减少MMP-2表达抑制大鼠肝纤维化。

Study of curcumin inhibition of hepatic fibrosis in rats

Objective:To investigate the effect of curcumin on hepatic fibrosis (HF) in rats and its molecular mechanism. Methods :Rat hepatic stellate cells were cultured and activated with ConA and processed with different concentration of curcumin. Models of hepatic fibrosis in rats induced by subcutaneous injection of CCl4 were treated with different concentrations of curcumin. The levels of HA, LN, PCⅢ and Ⅳ.C in serum and histological changes in liver tissues were determined to reveal the extent of liver fibrosis. Matrixmetalloproteinase-2 (MMP-2) expression in liver tissues and HSC was determined by Western blot and RT-PCR. Results:Curcumin significantly reduced the levels of HA, LN, PCⅢ and Ⅳ.C in serum of HF rats(P<0.05). the score of hepatic fibrosis in curcumin group was reduced significantly(P<0.05), Curcumin also inhibited MMP-2 expression in liver tissues(P<0.05) and activated HSC in a dose-dependent way(P<0.05). Conclusions:Curcumin can inhibit hepatic fibrosis in rats by reducing MMP-2 expression.