| 异氟醚和七氟醚后处理及JNK1/2通路变化对体外缺氧无糖损伤大鼠脑片的影响 |
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| 引用本文： | 王胜,代志刚,董希玮,郭素香,刘扬,江山,王志萍.异氟醚和七氟醚后处理及JNK1/2通路变化对体外缺氧无糖损伤大鼠脑片的影响[J].中国神经再生研究,2011,6(14):1091-1096. |
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| 作者姓名： | 王胜 代志刚 董希玮 郭素香 刘扬 江山 王志萍 |
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| 作者单位： | 新疆石河子大学医学院第一附属医院麻醉科，石河子 832008,新疆石河子大学医学院第一附属医院麻醉科，石河子 832008,新疆石河子大学医学院第一附属医院麻醉科，石河子 832008,新疆石河子大学医学院第一附属医院麻醉科，石河子 832008,新疆石河子大学医学院第一附属医院麻醉科，石河子 832008,江苏省麻醉医学研究所，江苏省麻醉学重点实验室，徐州221002,Department of Anesthesiology, Wuxi People’s hospital, Wuxi 214023, Jiangsu Province , China |
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| 基金项目： | 石河子大学自然科学基金专项（RCZX200688） |
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| 摘 要： | Recent research shows that the JNK1/2 signaling pathway plays a neuroprotective role against ischemia-reperfusion injury by cross-talk with other pathways.The present study investigated the effects of isoflurane and sevoflurane postconditioning on JNK1/2 pathway activity and neuronal cell viability after oxygen and glucose deprivation injury in hippocampal slices in vitro.Techniques used included population spike analysis,propidium iodide fluorescent staining,western blot assay,and the use of JNK1/2-specific pharmacological tools such as anisomycin (agonist) and SP600125 (inhibitor).We found that both isoflurane and sevoflurane inhibited JNK pathway activity and had neuroprotective effects against oxygen and glucose deprivation injury in slices of rat hippocampus in vitro.Postconditioning with volatile anesthetics exerted neuroprotective effects on nerve cells and preserved the function of the CA1 region by inhibiting JNK1/2 phosphorylation.This suppression of JNK1/2 activity could underlie the observed synergistic neuroprotective effect produced by volatile anesthetic postconditioning.
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| 关 键 词： | population spikes hippocampus oxygen and glucose deprivation volatile anesthetic postconditioning JNK1/2 neural regeneration |
| 修稿时间： | 2/9/2011 12:00:00 AM |
Effects of isoflurane and sevoflurane postconditioning and changes in JNK1/2 pathway activity on rat brain slices subjected to oxygen and glucose deprivation in vitro |
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| Sheng Wang,Zhigang Dai,Xiwei Dong,Suxiang Guo,Yang Liu,Shan Jiang and Zhiping Wang.Effects of isoflurane and sevoflurane postconditioning and changes in JNK1/2 pathway activity on rat brain slices subjected to oxygen and glucose deprivation in vitro[J].Neural Regeneration Research,2011,6(14):1091-1096. |
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| Authors: | Sheng Wang Zhigang Dai Xiwei Dong Suxiang Guo Yang Liu Shan Jiang and Zhiping Wang |
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| Institution: | 1Department of Anesthesiology,First Affiliated Hospital of Medical College,Shihezi University,Shihezi 832008,Xinjiang Uygur Autonomous Region,China 2Jiangsu Institute of Anesthesiology,Anesthesiology Key Laboratory of Jiangsu Province,Xuzhou 221002,Jiangsu Province,China3Department of Anesthesiology,Wuxi People’s Hospital,Wuxi 214023,Jiangsu Province,China |
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| Abstract: | Recent research shows that the JNK1/2 signaling pathway plays a neuroprotective role against ischemia-reperfusion injury by cross-talk with other pathways. The present study investigated the effects of isoflurane and sevoflurane postconditioning on JNK1/2 pathway activity and neuronal cell viability after oxygen and glucose deprivation injury in hippocampal slices in vitro. Techniques used included population spike analysis, propidium iodide fluorescent staining, western blot assay, and the use of JNK1/2-specific pharmacological tools such as anisomycin (agonist) and SP600125 (inhibitor). We found that both isoflurane and sevoflurane inhibited JNK pathway activity and had neuroprotective effects against oxygen and glucose deprivation injury in slices of rat hippocampus in vitro. Postconditioning with volatile anesthetics exerted neuroprotective effects on nerve cells and preserved the function of the CA1 region by inhibiting JNK1/2 phosphorylation. This suppression of JNK1/2 activity could underlie the observed synergistic neuroprotective effect produced by volatile anesthetic postconditioning.
Key Words: population spikes; hippocampus; oxygen and glucose deprivation; volatile anesthetic; postconditioning; JNK1/2; neural regeneration |
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| Keywords: | Population Spikes Hippocampal slice Anesthetic Postconditioning OGD JNK1/2 |
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