| Study on the migration of nerve stem cell in vitro Induced by glioma cells |
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| 作者姓名： | Yilu Gao Shuzi Gao Yi Fen Sheyu Lin Mei Liu |
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| 作者单位： | [1]Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong 226001, China [2]Key Laboratory of Nerve Regeneration, Nantong 226001,China |
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| 基金项目： | Nature Science Fundation of Jiangsu Province（BK2002145） |
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| 摘 要： | Objective： Previous studies have shown that glioma cells induced the migration of nerve stem cells （NSCs） in vivo. This study was exploring whether this situation could happen in vitro. Methods： Supematant from 05 glioma cell lines or astrocytes cultured in serum-free medium growing in logarithmic phase were separately placed in lower chambers and NSCs were placed in the upper chambers of Trans-well culture system. After 36 h co-incubation, these NSCs spheres occurred on the middle membrane were counted. Results： Results demonstrated that the supematant from 05 glioma cell culture, not from the astrocytes, enhanced the migration of NSCs （ P 〈0.01）. Conclusion： Some components in 05 glioma cell culture can attract the migration of NSCs.
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| 关 键 词： | 神经干细胞移植 神经胶质瘤 肿瘤细胞 病理机制 |
| 收稿时间： | 2005-07-19 |
Study on the migration of nerve stem cell in vitro Induced by glioma cells |
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| Yilu Gao,Shuzi Gao,Yi Fen,Sheyu Lin,Mei Liu.Study on the migration of nerve stem cell in vitro Induced by glioma cells[J].Acta Universitatis Medicinalis Nanjing,2005,19(6):325-328. |
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| Authors: | Yilu Gao Shuzi Gao Yi Fen Sheyu Lin Mei Liu |
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| Institution: | 1. Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong 226001, China
2. Key Laboratory of Nerve Regeneration, Nantong 226001, China |
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| Abstract: | Objective: Previous studies have shown that glioma cells induced the migration of nerve stem cells (NSCs) in vivo. This study was exploring whether this situation could happen in vitro. Methods: Supernatant from C6 glioma cell lines or astrocytes cultured in serum-free medium growing in logarithmic phase were separately placed in lower chambers and NSCs were placed in the upper chambers of Trans-well culture system. After 36 h co-incubation, these NSCs spheres occurred on the middle membrane were counted. Results: Results demonstrated that the supernatant from C6 glioma cell culture, not from the astrocytes, enhanced the migration of NSCs (P<0.01). Conclusion: Some components in C6 glioma cell culture can attract the migration of NSCs. |
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| Keywords: | neural stem cell glioma cell migration |
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