| 国产探针在荧光原位杂交FISH检测未培养羊水细胞性染色体中的应用 |
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| 引用本文： | 周荣生,丛林.国产探针在荧光原位杂交FISH检测未培养羊水细胞性染色体中的应用[J].安徽医学,2010,31(1):15-17,2. |
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| 作者姓名： | 周荣生 丛林 |
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| 作者单位： | 合肥市第三人民医院妇产科,230022;安徽医科大学第一附属医院妇产科 |
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| 摘 要： | 目的探讨国产探针在荧光原位杂交（FISH）检测孕中期未培养羊水中胎儿性染色体的应用价值并优化实验方法。方法选择CSPX／Y探针对15例孕周介于18～23周的孕妇的羊水标本进行FISH检测，同时对标本进行细胞培养和核型分析。结果14例标本出现满意的杂交信号，1例结果不满意。检测结果与染色体核型分析结果相符。针对实验中的不足，相应调整实验条件进行，获得了较满意的结果。结论国产探针实验结果理想，其有不足通过改进可以获得较满意的结果。
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| 关 键 词： | 荧光原位杂交 产前诊断 羊水 国产探针 |
The Application of domestic probe in detection of sexual chromosome in uncultured amniotic fluid by fluorescence in situ hybridization |
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| Zhou Rongsheng,Cong Lin.The Application of domestic probe in detection of sexual chromosome in uncultured amniotic fluid by fluorescence in situ hybridization[J].Anhui Medical Journal,2010,31(1):15-17,2. |
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| Authors: | Zhou Rongsheng Cong Lin |
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| Institution: | Zhou Rongsheng,Cong Lin Department of Obstetrics , Gynecology,the First Affiliated Hospital of Anhui Medicine University,Hefei 230022,China |
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| Abstract: | Objective To optimize the methods and evaluate the value of domestic probe in detection of sexual chromosome in uncultured amniotic fluid cells by fluorescence in situ hybridization (FISH).Methods FISH was performed with domestic centromeric probe (CSPX/Y) on the uncultured amniotic fluid samples those from 15 pregnant women between 18-23 weeks gestational.The cultured samples were analyzed concurrently by cytogenetics.Results Fourteen samples hybridization signals appear well.One sample hybridization signa... |
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| Keywords: | Automatic biochemical analyzer system Measurement Comparison Evaluation |
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