| 独脚金的高效液相色谱指纹图谱研究 |
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| 引用本文： | 方既明,章怀奋,黄松,赖小平. 独脚金的高效液相色谱指纹图谱研究[J]. 中国药师, 2010, 13(6): 800-802 |
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| 作者姓名： | 方既明 章怀奋 黄松 赖小平 |
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| 作者单位： | 1. 岳阳市一人民医院,湖南岳阳,414000
2. 广州中医药大学 |
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| 摘 要： | 目的：建立独脚金药材的指纹图谱分析方法.方法：采用反相高效液相色谱法,Dikma（DiamonsilTM C18（250 mm×4.6 mm,5μm）色谱柱,流动相为甲醇-2%甲酸水溶液系统,梯度洗脱,检测波长340 nm.结果：建立了独脚金药材的HPLC指纹图谱,确立了独脚金药材指纹图谱中的8个共有峰,计算出10批独脚金药材指纹图谱的相似度.结论：所建立的HPLC指纹图谱有很好的精密度、重复性和稳定性,能够用于独脚金药材的质量控制.
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| 关 键 词： | 独脚金 高效液相色谱 指纹图谱 |
Study on HPLC Fingerprint of Striga asiatiea(L.)O.Ktze |
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| Fang Jiming,Zhang Huaifen,Huang Song,Lai Xiaoping. Study on HPLC Fingerprint of Striga asiatiea(L.)O.Ktze[J]. China Pharmacist, 2010, 13(6): 800-802 |
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| Authors: | Fang Jiming Zhang Huaifen Huang Song Lai Xiaoping |
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| Affiliation: | 1. No. 1 People＇s Hospital of Yueyang, Hunan Yueyang 414000, China ;2. Guangzhou University of TCM) |
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| Abstract: | Objective： To establish an HPLC fingerprint analysis method of Striga asiatica （L.） O. Ktze. Method： Chromatographic fingerprint of Striga asiatica （L.） O. Ktze. was investigated by RP-HPLC. The chromatographic column was Dikma （ DiamonsilTM C18（250 mm ×4. 6 mm,5 μm）. The mobile phase consisted of methanol-2% formic acid and the gradient elution mode was ap- piled to chromatographic separation. The detection wavelength was 340 nm. Result： The HPLC fingerprint of Striga asiatica （L.） O. Ktze. was established preliminarily, 8 characteristic peaks were selected and the similarity among 10 batches of Striga asiatica （L.） O. Ktze. from different aera were determined. Conclusion： HPLC fingerprint method is accurate, repeatable, stable, and can be used for quality control of Striga asiatica （ L. ） O. Ktze. |
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| Keywords: | Striga asiatica （ L. ） O. Ktze. HPLC Fingerprint |
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