核干细胞因子mRNA在原代白血病细胞中的表达

【目的】探讨急性白血病（AL）患者骨髓单个核细胞（BMNC）核干细胞因子（NS）基因的表达水平。【方法】采用半定量RT—PCR方法检测52例AL患者及20例骨髓正常的非恶性血液病（对照）的BMNCNS基因的表达水平。【结果】在52例AL患者的BMNC中均存在NS基因的表达，20例非恶性血液病对照的BMNC极低表达或不表达NS基因。急性淋巴细胞白血病患者和急性非淋巴细胞白血病患者NSmRNA的相对表达量均明显高于对照组（P〈0．01）。但在AL各亚型之间NSmRNA的表达无明显差异（P=0．253），NSmRNA的表达水平与患者初诊时骨髓原始细胞数呈正相关，与外周血白细胞数及血小板数无关。【结论】Ns基因在AI。原代细胞中表达上调，但NSmRNA表达水平与AL患者某些临床特征无相关性。

Expression of Nucleostemin mRNA in Primary Human Leukemia Cells

Objective]To evaluate the expression of nucleostemin（NS） gene in bone marrow mononuclear cells （BMNC） from adult patients with acute leukemia（AID. Methods] The bone marrow mononuclear cells obtained from 52 patients with Air and 20 normal bone marrow subjects as controls were separated by density gradient centrifugation. RT-PCR was used to detect the NS expression in patients with AL and normal hone marrow subjects. Results] The expression of NS mRNA in BMNCs from 52 patients with AL was detected, and no expression or only the extremely weak expression was detected in 20 subjects. The mRNA relative ex- pression of NS in patients with AL was higher than that in the control group, and the difference was significant （ P 〈0. 001）, but there was no significant difference in acute lymphoblastie leukemia（ALL）, M1, M2, M3, M4 and M5 subtypes of acute non lymphoblastic leukemia（ANLL）（ P = 0. 253）. The mRNA expression of NS gene in patients with AL at onset was correlated with blast cells rates in bone marrow cells, and was not correlated with platelets and white blood cells counts in total peripheral blood. Conclusion]The expression of NS gene is up regulated in AL cells. But the mRNA expression level of NS gene is not correlated with some clinical characters of patients with AL at onset.