丹参复合微乳抗垂体后叶素致大鼠急性心肌缺血的药效学研究

目的探讨丹参复合微乳(Co-ME)抗垂体后叶素致大鼠急性心肌缺血的药效。方法采用舌下静脉注射垂体后叶素造成心肌缺血的方法,检测丹参复合微乳和空白微乳、丹参酮微乳、丹酚酸微乳、丹参酮混悬液各给药组在注射垂体后叶素30min后血清中磷酸肌酸激酶(PCK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)的含量。结果丹参复合微乳、丹参酮微乳、丹酚酸微乳和丹参酮混悬液均可在一定程度上改善注射垂体后叶素诱导的急性心肌缺血,减少PCK、LDH活性及MDA的生成量、提高心肌组织SOD活性,且以丹参复合微乳疗效最为显著,具有优于丹参酮微乳、丹酚酸微乳的作用趋势,并优于丹参酮混悬液。结论微乳载药体系可明显促进丹参酮的吸收,提高生物利用度,对急性心肌缺血具有治疗作用,丹参酮和丹酚酸复合可增加疗效。

Pharmacodynamics of Danshen co-microemulsion in treatment of experimental acute myocardial ischemia induced by pituitrin in rats

ObjectiveTo study the effects of Danshen co-microemulsion (Co-ME) in treatment of acute myocardial ischemia induced by pituitrin in rats. MethodsThe experimental model of myocardial ischemia was produced by injecting pituitrin into the sublingual vein of rats. The serum level of malonaldehyde(MDA) and the activities of phosphocreatine kinase (PCK) , lactate dehydrogenase (LDH), and erythrocuprein (SOD) were examined in Co-ME, salvianolic acid microemulsion, tanshinone microemulsion, tanshinones suspension and blank microemulsion groups 30 min after injection of pituitrin. ResultsCo-ME, tanshinone microemulsion, salvianolic acid microemulsion and tanshinone suspension all improved myocardial eschemia to some extent; besides, they also decreased PCK, LDH activities and MDA content, and increased SOD activity. The therapeutic effect of Co-ME group was superior to those for the tanshinone microemulsion and salvianolic acid microemulsion groups, and significantly better than that of tanshinone suspension group (P<0.05). ConclusionMicroemulsion system can greatly promote the absorbance and bioavailability of tanshinone, displaying a therapeutic effect for acute myocardial ischemia. Salvianolic acids combined with tanshinones can promote the therapeutic effect.