| RAPD法鉴定射干类中药 |
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| 引用本文： | 黄芸,秦民坚,杨光,徐珞珊,周开亚.RAPD法鉴定射干类中药[J].医学教育探索,2002(10):935-937. |
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| 作者姓名： | 黄芸 秦民坚 杨光 徐珞珊 周开亚 |
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| 作者单位： | [1]中国药科大学中药学院,江苏南京210038 [2]南京师范大学江苏省资源生物技术重点实验室,江苏南京210097 |
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| 基金项目： | 国家自然科学基金(3 0 170 10 3 ),江苏省资源生物技术重点实验室资助项目,江苏省自然科学基金资助项目(BK970 85 ) |
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| 摘 要： | 目的：用RAPD技术对射干类药材射干Belamcanda chinensis及混淆品鸢尾属鸢尾Iris tectorum、野鸢尾I．dichotoma、蝴蝶花I．japonica、德国鸢尾I．germanica等5种药用植物进行分子水平的鉴定。方法：CTAB法和DNeasy^TM Plant Mini Kit法提取射干类药材5种原植物总DNA,以随机引物进行随机扩增。结果：用OPD－08（5’-gtgtgcccca-3‘）等作随机扩增引物进行随机扩增时,可得到识别这些物种基因组DNA的多态片段。结论：RAPD法能有效的鉴别射干类药材,把射干和鸢尾、野鸢尾、蝴蝶花、德国鸢尾等药用植物区分开。
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| 关 键 词： | 射干 鸢尾属 RAPD DNA指纹图谱 药材鉴定 |
Identification of Belamcanda chinensis and related Chinese materia medica of Iris L. by RAPD analysis |
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| HUANG Yun,QIN Min jian,YANG Guang,XU Luo shan,ZHOU Kai ya.Identification of Belamcanda chinensis and related Chinese materia medica of Iris L. by RAPD analysis[J].Researches in Medical Education,2002(10):935-937. |
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| Authors: | HUANG Yun QIN Min jian YANG Guang XU Luo shan ZHOU Kai ya |
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| Abstract: | Object To indentify the crude drugs of "Shegan" Belamcanda chinensis (L.), DC.] and related Chinese materia medica of and related Chinese materia medica of Iris L., including Iris tectorum Maxim., I. dichotoma Pall., I. germanica L. and I. japonica Thunb. by RAPD technique. Methods General DNA was isolated from fresh leaves of B. chinensis and five plants of Iris L. by CTAB and DNeasy TM Plant Mini Kit. The random amplified polymorphic DNA (RAPD) was used to identify them. Results The polymorphic feather of DNA fingerprints of different species were compared by primer OPD 08. The studies showed that they have different relationships in heredity and the method of RAPD can be used to identify them easily. Conclusion Chinese materia medica of Iris L. can be idenlified by RAPD, and B. chinensis could be differentiated from I. tectorum, I. dichotoma, I. japonica, I. germanica. |
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| Keywords: | Belamcada chinensis (L ) DC Iris L RAPD DNA fingerprints |
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