瑞香狼毒水提物小鼠药物血清对小鼠白血病L1210细胞增殖、克隆形成和DNA合成的影响

目的；从整体水平探讨瑞香狼毒水提物（SCLA）的抗癌作用及机制。方法：以不同剂量SCLAig给药后，不同时间采集小鼠血清，处理体外培养的小鼠白血病L1210细胞，观察肿瘤细胞MTT还原，克隆形成能力和DNA合成的改变。结果；SCLA3，6，12g/kg给药1，2，4，8h后的药物血清处理肿瘤细胞后，其MTT还原及克隆形成率均显著降低；给药2h时的药物血清表现出更强的抑制肿瘤细胞增殖作用。SCLA小鼠药物血清也显著抑制^3H]TdR掺入L1210细胞DNA。结论：直接抑制癌细胞增殖及DNA合成是瑞香狼毒的重要抗癌机制。

Effect of serum Ruixiang Langdu (Stellera chamaejasme) extract on proliferation,clonal formation and DNA synthesis of mouse L1210 leukemic cells

Object To explore, on the whole, the anticancer activity and mechanism of the aqueous extract of Ruixiang Langdu (Stellera chamaejasme L.) (SCLA). Methods Mice were given different i.g. doses of SCLA and their serum collected at different intervals after drug administration. The effects of the serum on the proliferation of mouse L 1210 leukemic cells were observed with MTT assay, clone formation and incorporation of 3H] TdR into DNA of the cells. Results Serum SCLA collected 1, 2, 4, 8 h after administration of 3, 6, and 12 g/kg SCLA showed significant decrease of MTT formazans and clone formation. Serum SCLA collected 2 h after drug administration showed more strong inhibition of cancer cell proliferation. It also inhibited the incorporation of 3H] TdR into DNA of L 1210 cells. Conclusion SCLA showed a remarkable inhibitory effect on proliferation and DNA synthesis of cancer cells cultured in vitro, which may be the main anticancer mechanisms of SCLA.