改良冻融制备成年兔周围神经去细胞材料的研究

目的:探讨兔坐骨神经去细胞材料的组织形态学,为组织工程化周围神经研究提供支架材料。方法:切取健康成年新西兰兔两侧坐骨神经各2 cm,分别按传统冻融、改良冻融、未处理3种方法制备材料,分别行大体形态、光镜、透视电镜检查以及基膜管lamina成分观察和分析。结果:改良冻融方法制备的材料细胞成分去除更加彻底,神经基膜管通畅,lamina成分保留完好。结论:改良冻融方法制备获得的去细胞材料,可能是具有仿生结构的组织工程化神经支架较为理想的材料。

Preparation of acellular materials with adult rabbits' peripheral nerve by improved freeze thaw

Objective：To study the histomorphology of the acellular materials from rabbits＇ sciatic nerve and to provide materials for the study of tissue engineering peripheral nerve. Methods： The bilateral sciatic nerve of 36 healthy adult New Zealander rabbits was cut 2 em and aeellular materials were prepared by traditional freeze thaw, improved freeze thaw and no-freeze thaw ; the above materials were examined by configuration, light microscope and electron microscope; base membrane pipe lamina composition was observed and analyzed. Results： The cell composition of the materials made by the improved method was thoroughly removed, the nerve base membrane was retained and the reservation of lamina composition was intact. Conclusions ： The aeellular materials prepared by improved freeze thaw method may be ideal for building tissue engineering nerve.