| HBsAg阳性孕妇血清中乙肝病毒前S/S基因的相似株现象 |
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| 引用本文： | 李端,闫永平,徐德忠,张景霞.HBsAg阳性孕妇血清中乙肝病毒前S/S基因的相似株现象[J].第四军医大学学报,2001,22(12):1122-1124. |
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| 作者姓名： | 李端 闫永平 徐德忠 张景霞 |
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| 作者单位： | 第四军医大学预防医学系流行病学教研室, |
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| 基金项目： | 国家自然科学基金资助项目 !(3 9970 62 5 ) |
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| 摘 要： | 目的：探讨HBsAg阳性孕妇体内HBV病毒前S/S基因变异的状况。方法：应用全长PCR技术从4例表面抗原阳性孕妇血清中扩增出HBV全长基因，将其克隆于pUC19载体，每例随机筛选5个阳性克隆，对其前S/S区进行序列分析。结果：4例患各克隆间均存在碱基的差异，此类患体内HBV株同源性较差。结论：HBsAg阳性孕妇体内HBV株呈“相似株”分布。
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| 关 键 词： | 乙型肝炎病毒 表面抗原阳性 前S／S基因 相似株 |
| 文章编号： | 1000-2790(2001)12-1122-03 |
| 修稿时间： | 2001年2月13日 |
Quasispecies of HBV preS/S gene in HBsAg carrying pregnant women |
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| LI Duan,YAN Yong-ping,XU De-Zhong,ZHANG Jing-Xia.Quasispecies of HBV preS/S gene in HBsAg carrying pregnant women[J].Journal of the Fourth Military Medical University,2001,22(12):1122-1124. |
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| Authors: | LI Duan YAN Yong-ping XU De-Zhong ZHANG Jing-Xia |
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| Abstract: | AIM To study the mutation of HBV preS/S gene in HBsAg carrying pregnant women. METHODS Full length polymerase chain reaction (PCR) was used to amplify whole HBV gene from sera of 4 HBsAg carrying pregnant women. The full length PCR products were cloned into pUC19 vector,5 clones were obtained every woman and subgenomic PCR was used to amplify HBV preS/S gene for DNA sequencing. RESULTS Compared with the wild type HBV C, all clones exhibited base insertion and substitution. CONCLUSION Quasispecies are the major characteristics in these HBsAg carrying pregnant women. |
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| Keywords: | hepatitis B virus preS/S gene quasispecies variation (genetics) |
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