哮喘小鼠C_7～T_5节段脊髓后角BDNF及其受体TrkB的表达研究

目的探讨在哮喘发病中,脑源性神经营养因子(BDNF)及其高亲和力受体酪氨酸激酶B(TrkB)在哮喘小鼠C7～T5节段脊髓后角内的表达变化。方法BALB/c小鼠20只,按随机数字表法均分为正常对照组和哮喘组,利用AniRes2005肺功能仪测小鼠气道阻力、免疫荧光方法和Western blot方法检测各组小鼠C7～T5节段脊髓后角内BDNF及其高亲和力受体TrkB的表达变化。结果哮喘组小鼠吸气阻力和呼气阻力明显高于正常组(P<0.01),哮喘模型建立成功。免疫荧光结果显示哮喘组C7～T5节段脊髓后角内BDNF及TrkB阳性产物的平均光密度值(MOD)显著高于正常对照组(P<0.01),Western blot方法检测也得到了相同的结果。结论哮喘小鼠C7～T5节段脊髓后角内BDNF及TrkB的表达升高。

The expression of BDNF and TrkB in the spinal dorsal horn of the asthmatic mice

Objective To study the expression of brain-derived neurotrophic factor(BDNF) and its high-affinity receptor tyrosine kinase receptor B(TrkB) in the spinal dorsal horn of the asthmatic mice.Methods 20 BALB/c mice were randomly divided into normal control group and asthmatic group.The airway resistance of mice was measured with AniRes 2005 pulmonary function meter;The expressions of BDNF and TrkB were observed by immunofluorescence and western blot methods of all the groups.Results Inspiratory resistance and expiratory resistance in the asthmatic mice were obviously higher than in normal control mice,the asthmatic model was successfully constructed.The mean optic density(MOD) of BDNF and TrkB positive product in the C7-T5 spinal dorsal horn increased significantly in the asthmatic mice than in normal control by means of immunofluorescence(P<0.01),and the same result was obtain by western blot method.Conclusion The expressions of BDNF and TrkB were elevated in the C7-T5 spinal dorsal horn of the asthmatic mice.