LY-294002对大鼠急性脊髓损伤后Caspase-3表达的影响

目的研究LY-294002对急性脊髓损伤大鼠Caspase-3表达的变化。方法选取104只成年健康SD大鼠,按Nystrom法建立大鼠脊髓(T8～9)急性压迫损伤模型,按随机数字表法分为假手术组8只、损伤组48只、LY-294002组48只。免疫组化和Western blot方法检测各组大鼠脊髓损伤部位Caspase-3的表达。结果免疫组化结果发现假手术组大鼠脊髓神经细胞中Caspase-3阳性细胞较少;在脊髓损伤后8 h时,Caspase-3阳性细胞数明显增多,3 d达高峰,7 d表达开始减弱。在经LY-294002处理后的各时间点大鼠脊髓损伤部位的Caspase-3阳性细胞明显少于相同时间点的脊髓损伤组大鼠。Western blot方法发现假手术组大鼠脊髓损伤部位的Caspase-3的表达量很少,而在脊髓损伤后8h时,Caspase-3蛋白表达量明显增多,3 d达高峰,7 d表达开始减弱,在经LY-294002处理后的各时间点大鼠脊髓损伤部位的Caspase-3蛋白表达则明显少于相同时间点的脊髓损伤组大鼠,与免疫组化结果一致。结论LY-294002能够下调急性脊髓损伤大鼠Caspase-3蛋白的表达。

The effect of LY294002 on the expression of Caspase-3 after acute spinal cord injury

Objective To study the effect of LY294002 on the expression of Caspase-3 after acute spinal cord injury. Methods 104 SD healthy adult rats were chose,and randomly divided into the sham group(8),the spinal cord injury group(48),LY-294002 group(48) on average.The spinal cord injury(SCI) was induced with Nystrom's way by the moderate compression at the level of T8 and T9 spinal cord.The expression of Caspase-3 was observed by immunochemistry and western blot methods. Results There were few expression of Caspase-3 positive cells in sham group by immunohistochemical study.The number of positive cells of Caspase-3 was increased obviously at 8 hours,got to a peak at 3 days,and was lowest at 7 days.Compared with SCI group,The number of positive cells of Caspase-3 at the same time point was significantly lower in injury sites of rats in LY-294002 group.There were few expression of Caspase-3 in sham group by western blot.The expression of Caspase-3 was increased obviously at 8 hours,got to a peak at 3 days,and was lowest at 7 days.Compared with SCI group,The expression of Caspase-3 at the same time point was significantly lower in injury sites of rats in LY-294002 group,and agree to the result of immunochemistry.Conclusion LY-294002 could downregulate the expression of Caspase-3 of rat after acute spinal cord injury