DNA polymorphism of HLA class II genes in systemic lupus erythematosus |
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Authors: | Jack B. Cowland Vagn Andersen Poul Halberg Niels Morling |
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Affiliation: | Institute of Forensic Genetics, University of Copenhagen;Department of Rheumatology, University Hospital (Rigshospitalet);Department of Rheumatology, Hvidovre Hospital, Copenhagen, Denmark |
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Abstract: | Abstract: We investigated the DNA restriction fragment length polymorphism (RFLP) of the major histocompatibility complex (MHC) genes: HLA-DRB, -DQA, -DQB, -DPB in 24 Danish patients with systemic lupus erythematosus (SLE) and in 102 healthy Danes. A highly significant increase of the frequency of the DR3- and DRw6-associated 7.00 kb DRB Taq I DNA fragment was found in SLE patients compared to normal controls (83.3% vs 35.5%; RR = 9.1, p < 10-4). The frequencies of the DQA1*0501-associated 4.56 kb DQA Taq I fragment and the DRB3*01/03-associated 9.79 kb Taq I fragment were also found to be significantly increased in SLE patients (70.8% vs 29.7%; RR = 5.8, p < 10-2 for the DQA fragment and 70.8% vs 36.1%; RR = 4.3, p < 0.05 for the DRB3 fragment). Less extensive and insignificant increases of the frequencies of the DR3-associated DQB and DPB fragments were observed. The frequencies of the DR2-associated DRB, DQA, and DQB fragments were comparable to those found in normal controls. |
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Keywords: | Danes DNA polymorphism HLA SLE |
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