阴道加德纳菌致病株的检测

应用聚合酶链反应（ＰＣＲ）法检测阴道加德纳菌（Ｇ．Ｖａｇ）致病株，探讨其临床诊断价值。在ＩＴＳ－２３ｓｒＲＮＡ基因上设计一对特异性引物Ｐ１／Ｐ２，建立ＰＣＲ反应体系，特异地扩增Ｇ．Ｖａｇ致病株。结果Ｇ．Ｖａｇ扩增片段４３３ｂｐ，１４１例有临床症状者检出３６例阳性，阳性率２５５％，１２９例无症状对照组检出６例阳性，阳性率４６％，两者比较差异有显著性。该ＰＣＲ反应体系特异地检测Ｇ．Ｖａｇ致病株，为临床诊断及其致病性的研究提供了新的检测方法

PCR in detection pathogenic gardnerella vaginalis

In order to explore the clinical diagnostic values of pathogenic gardnerella vaginalis (GV) detected by PCR. We designed a pair of specific prime according to the sequencing in ITS-23srRNA gene and specifically establish a PCR method to detect gardnerella vaginalis. This PCR method can amplify a 433bp zone of GV specifically. 141 samples of patients with clinical symptoms and 129 cases without clinical symptom were detected by this method, samples with positive results were 36 and 6, respectively. So the positive rate was significantly difference between the two groups (25 5% VS. 4 6%). This method can specifically detect GV, and may be a new method for the clinical diagnosis and pathogenic research of GV.