3-硝基丙酸预处理诱导沙土鼠脑缺血耐受与海马星形胶质细胞的激活

目的：探讨海马区星形胶质细胞的激活与3－硝基丙酸（3－NPA）预处理诱导脑缺血耐受的关系。方法：阻断沙土鼠双侧颈总动脉造成前脑缺血模型，通过HE染色和免疫组化观察海马锥体细胞死亡和星形胶质细胞的反应。结果：对照组海马CA1区已失去正常结构，锥体细胞大部分丧失，存活神经元计数显低于假手术组。3－NPA预处理组存活神经元减少，但高于对照组，假手术组海马CA1区仅见少量胶质原纤维酸性蛋白（GFAP）阳性细胞，染色较弱，突起不明显，对照组海马CA1区GFAP阳性细胞增多，多为弱阳性。3－NPA预处理组海马CA1区GFAP阳性细胞数目明显增多，染色较深，突起增粗。结论：星形胶质细胞形态和机能的改变可能与3－硝基丙酸预处理诱导脑缺血耐受有关。

Relation between activation of hippocampal astrocytes and ischemic tolerance induced by 3-nitropropionic acid in gerbils

AIM: To investigate the relationship between astroglial activation state and ischemic tolerance induced by low dose of 3 nitropropionic acid (3 NPA) in gerbil hippocampus. METHODS: Transient forebrain ischemic model was induced by bilateral common carotid arteries occlution. HE staining and immunohistochemistry were used to identify neuronal and astrocyte response. RESULTS: Preconditioning with 3 NPA produced protective effects of CA 1neurons. The number of glial fibrillary acidic protein positive astrocyte in hippocampal CA 1 region increased slightly in control group, but increased significantly in preconditioning of the brain with 3 nitropropionic acid. CONCLUSION: The state of astroglial activation is related to neuronal survival in ischemic tolerance induced by low dose of 3 nitropropionic acid.