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| 自配试剂在COULTER流式细胞仪检测NK细胞和B细胞中的应用 | |
| 引用本文： | 徐明辉,陈智平,秦雪,莫武宁,王立秋,李若林,林发全,黄军强,李山. 自配试剂在COULTER流式细胞仪检测NK细胞和B细胞中的应用[J]. 标记免疫分析与临床, 2009, 16(2) |
| 作者姓名： | 徐明辉 陈智平 秦雪 莫武宁 王立秋 李若林 林发全 黄军强 李山 |
| 作者单位： | 1. 广西医科大学第一附属医院临床实验部,广西医科大学医学检验系,广西,南宁,530021 2. 广西医科大学公共卫生学院,广西,南宁,530021 3. 哈尔滨医科大学第五附属医院检验科,黑龙江,大庆,163316 |
| 摘 要： | 探讨美国COULTER流式细胞仪试剂国产化问题.将自配全血溶解试剂、鞘液及清洗液的理化性质、精密度、准确度、稳定性与原装试剂进行对比分析.分析结果显示自配试剂与原装试剂理化性质一致,采用自配试剂测定20份病人标本NK细胞和B细胞并与原装试剂对比,差异无统计学意义(P＞0.05),两者呈高度正相关r＞0.95.自配试剂稳定性好,放置1年后,与原装试剂分别测定15份标本,结果差异无统计学意义(P＞0.05),两者呈高度正相关r＞0.91,说明自配试剂完全可以代替美国COULTER流式细胞仪原装进口试剂进行NK细胞和B细胞的检测. |
| 关 键 词： | 流式细胞仪 试剂研制 NK细胞 B细胞 |
Application of Self-made Reagent in the Detection of NK Cells and B Cells by Beckman-Coulter Flow Cytometer |
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| XU Ming-hui,CHEN Zhi-ping,QIN Xue,MO Wu-ning,WANG Li-qiu,LI Ruo-lin,LIN Fa-quan,HUANG Jun-qiang,LI Shan. Application of Self-made Reagent in the Detection of NK Cells and B Cells by Beckman-Coulter Flow Cytometer[J]. Labeled Immunoassays and Clinical Medicine, 2009, 16(2) | |
| Authors: | XU Ming-hui CHEN Zhi-ping QIN Xue MO Wu-ning WANG Li-qiu LI Ruo-lin LIN Fa-quan HUANG Jun-qiang LI Shan |
| Affiliation: | XU Ming-hui,CHEN Zhi-ping,QIN Xue,MO Wu-ning,WANG Li-qiu,LI Ruo-lin,LIN Fa-quan,HUANG Jun-qiang,LI Shan(Department of Clinical Laboratory,First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
| Abstract: | To explore the localization issues of reagents used in Beckman-Coulter Flow Cytometer,the self-made haemolysis reagent,sheath liquid and cleansingsolution were used to detect NK cells and B cells in 20 patients samples by Beckman-Coulter Flow Cytometer,their physicochemical properties,precisions,accuracy and stability were compared with import reagents.The results showed that the physicochemical properties of self-made reagents were those of import reagents.There were no significantly difference between sel... |
| Keywords: | Flow Cytometer Reagent NK cells B cells |
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