岩大戟内酯B诱导乳腺癌MCF-7细胞凋亡的实验研究

目的：探讨岩大戟内酯B 对MCF-7 乳腺癌细胞生长抑制作用及其机理.方法：通过MTT 法观察各组细胞增殖能力;采用流式检测技术分析细胞周期;利用Annexin V/PI 双染检测细胞凋亡情况;电镜观察细胞形态.结果：随着岩大戟内酯B 浓度增加对MCF-7 的抑瘤率增高;G2/M 期细胞减少,S 期细胞增多,出现S 期阻滞;晚期凋亡和死亡的细胞增加;电镜显示加药后细胞出现核固缩,细胞器空泡变性,凋亡小体形成,甚至出现坏死.结论：岩大戟内酯B 对乳腺癌MCF-7 细胞生长明显抑制作用,并诱导肿瘤细胞凋亡,为临床开发新药提供理论依据.

The Experiment Research on Apoptosis of Breast Cancer Cell Line MCF-7 Treatment with Jolkinolide B

Objective： To investigate the inhibition effects of Jolkinolide B on breast cancer cell growth inhibition and its mechanism. Methods： Author used MTT method to observe the proliferation of MCF-7 cell lines when treat with Jolkinolide B; Cell cycle was assayed by flow cytometer; Annexin V/PI double dye was detected to cell apoptosis; Cell morphology was observed by electronic microscopy. Results： The inhibit ratios of MCF-7 cell lines were increased following the drug concentrations of Jolkinolide B; The G2 / M period cells reduce, S phase increased the number of cells and increase apoptosis rates, it appears the block of S phase; There were a lot of apoptotic cell and dead cell; Many nuclei had become pyknotic by electronic microscopy, organelles empty bubble degeneration, appear apoptotic body, even necrosis after dosing the different concentration of drug. Conclusion： Jolkinolide B can significantly inhibit the growth of MCF-7 breast cancer cell and induced apoptosis. It is provides the theory for a new drug with the development of clinical therapy.