Co-cultured transformed and untransformed C3H 10T1/2 cells: preferential killing of transformed cells by low dose rate irradiation |
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| Authors: | E M Zeman J S Bedford |
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| Affiliation: | 1. Teaching and Research Section of Nuclear Medicine, School of Basic Medical Sciences, Anhui Medical University, 230032, Hefei, China;2. North China University of Water Resources and Electric Power, 450045, Zhengzhou, China;3. Institute of Nuclear Energy Safety Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, 230031, Hefei, China;4. Institute of Energy, Hefei Comprehensive National Science Center, 230031, Hefei, China;5. Department of Nuclear Science and Technology, Nanjing University of Aeronautics and Astronautics, 210016, Nanjing, China;1. Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran;2. Department of Nuclear Engineering, School of Mechanical Engineering, Shiraz University, Shiraz, Iran;3. MVLS College, The University of Glasgow, Glasgow Scotland, UK;4. Physiology Department, School of Medicine, Guilan University of Medical Sciences, Guilan, Iran;5. Department of Radiation Dosimetry, Nuclear Physics Institute of the CAS, Prague, Czechia;6. Technische Universität Wien, Atominstitut, Vienna, Austria |
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| Abstract: | Co-cultured C3H 10T1/2 cells, in which transformed cells were grown as discrete colonies on top of density-inhibited monolayers of untransformed cells, were used to determine the potential usefulness of a short term assay system for the study of differential radiation effects as they may apply to cell populations with differing turnover rates, but in close physical contact. Mixed cultures were exposed to either an acute dose of 20 Gy of Cs-137 gamma rays, or a dose of 72 Gy delivered at a low dose rate of 0.34 Gy per hour. These treatments resulted in approximately equal levels of damage to the untransformed monolayers. At 10-day intervals after treatment, representative flasks from each dose group were examined for evidence of degeneration, and subsequent regrowth, if any, of the transformed colonies and untransformed monolayers. For comparable amounts of visible damage to the untransformed monolayers, the low dose rate irradiation was more effective at delaying regrowth of, or even eradicating, transformed colonies. These results are consistent with expectations based on previous results in which dose-rate or dose fractionation isoeffect curves were compared for these two cell types, grown independently in plateau phase cultures. |
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