大肠癌患者血浆DNA中K-ras癌基因突变的检测 |
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引用本文: | 何晓文,李峻,靳小青,卢建,孙民,钮燕,陆翠琴. 大肠癌患者血浆DNA中K-ras癌基因突变的检测[J]. 中华消化杂志, 2002, 22(1): 26-28 |
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作者姓名: | 何晓文 李峻 靳小青 卢建 孙民 钮燕 陆翠琴 |
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作者单位: | 1. 200433,上海,海军医学研究所新药研究中心 2. 第二军医大学病理生理教研室 3. 解放军第四一一医院外科 |
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摘 要: | 目的 评价血浆DNA中K-ras癌基因第12密码子点突变作为肿瘤标记物的临床应用价值。方法 用引物序列特异性聚合酶扩增链式反应(PASP)检测了32例大肠癌患者肿瘤组织DNA、血浆DNA中K-ras癌基因第12密码子点突变,对所有PASP法扩增得到的含点突变的PCR产物进行Sanger双脱氧链终止法测序。结果 14例(44%)大肠癌肿瘤组织DNA中存在K-ras癌基因第12密码子点突变;其中的13例(93%)在血浆DNA中存在与其肿瘤组织DNA相同类型的基因点突变。对于18例肿瘤标本DNA未见突变的各期大肠癌及5例正常献血员对照组,在其血浆DNA中也无一发现K-ras基因突变。结论 血浆DNA中K-ras癌基因第12密码子点突变有望成为一个肿瘤标志物应用于大肠癌的诊断。
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关 键 词: | 大肠癌 K-ras癌基因点突变 血浆DNA 聚合酶扩增链式反应 |
修稿时间: | 2001-01-12 |
Detection of K-ras mutation in DNA extracted from the plasma of patients with colorectal cancer |
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Abstract: | Objective The purpose of this study was to determine the value of K-ras mutation in DNA extracted from the plasma as clinical indicator of colorectal cancer. Methods Point mutation at codon 12 of K-ras gene was assayed by polymerase chain reaction of sequence-specific primers in DNA extracted from the plasma and tumors from 32 patients with colorectal cancer. The mutation was further confirmed by dideoxy-mediated chain-termination method of DNA sequencing. Results Fourteen patients (44%) had a codon 12 K-ras mutation within their primary tumors and identical mutations were found in the plasma DNA of 13 patients (93%). Mutant DNA was not detected in the plasma specimens of 18 patients whose tumors tested negative for K-ras alterations or in 5 healthy control subjects. Conclusion Preliminary results suggest the detection of K-ras mutations in circulating DNA extracted from the plasma specimens may have some clinical uti- lity in the detection of colorectal cancer. |
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Keywords: | Colorectal cancer K-ras mutation DNA extracted from the plasma Polymerase amplification chain reaction of sequence-specific primers |
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