| HPLC测定贯叶金丝桃滴丸中金丝桃苷含量 |
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| 引用本文： | 曹广尚,杨培民,李芳.HPLC测定贯叶金丝桃滴丸中金丝桃苷含量[J].中国中医药信息杂志,2014(7):67-68. |
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| 作者姓名： | 曹广尚 杨培民 李芳 |
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| 作者单位： | 山东中医药大学附属医院,山东济南250011 |
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| 基金项目： | 国家科技重大专项一重大新药创制（2009ZX09102-142）：山东省科技发展计划（2012GGB14018） |
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| 摘 要： | 目的建立高效液相色谱法测定贯叶金丝桃滴丸中金丝桃苷含量的方法。方法采用Kromasil C18色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸(16∶84),检测波长为360 nm,流速1.0 mL/min,柱温30℃。结果金丝桃苷在0.132~1.188μg范围内线性关系良好(r=0.999 9),平均加样回收率为99.55%(RSD=0.78%)。结论本方法简便、准确、可靠,可用于贯叶金丝桃滴丸中金丝桃苷的质量控制。
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| 关 键 词： | 贯叶金丝桃滴丸 金丝桃苷 高效液相色谱法 |
Content Determination of Hypericin in Guanye Jinsitao Drop Pills by HPLC |
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| CAO Guang-shang,YANG Pei-min,LI Fang.Content Determination of Hypericin in Guanye Jinsitao Drop Pills by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2014(7):67-68. |
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| Authors: | CAO Guang-shang YANG Pei-min LI Fang |
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| Institution: | (Affiliated Hospital of Shandong University of TCM, Jinan 250011, China) |
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| Abstract: | Objective To build a method for content determination of Hypericin in Guanye Jinsitao drop pills by HPLC.Methods The column was Kromasil C18 （150 mm×4.6 mm, 5μm）, mobile phase was acetonitrile-0.1% phosphoric acid （16∶84）, UV detection was performed at 360 nm, the flow rate was 1.0 mL/min, and the detector drift tube temperature was set at 30℃.Results The calibration curves were linear in the range of 0.132-1.188μg for Hypericin （r=0.999 9）. The average recovery was 99.55% （RSD=0.78%）.Conclusion The method is simple, accurate, reliable, and can be used for the quality control of Hypericin in Guanye Jinsitao drop pills. |
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| Keywords: | Guanye Jinsitao drop pills Hypericin HPLC |
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