| HPLC测定舒通安泰胶囊中小檗碱、表小檗碱、黄连碱、巴马汀含量 |
| |
| 引用本文： | 侯建忠,喻利坚,杨铖. HPLC测定舒通安泰胶囊中小檗碱、表小檗碱、黄连碱、巴马汀含量[J]. 中国中医药信息杂志, 2014, 0(7): 61-63 |
| |
| 作者姓名： | 侯建忠 喻利坚 杨铖 |
| |
| 作者单位： | [1]酒泉市药品检验检测中心,甘肃酒泉735000 [2]酒泉市中医院,甘肃酒泉735000 |
| |
| 摘 要： | 目的建立舒通安泰胶囊中小檗碱、表小檗碱、黄连碱、巴马汀的高效液相色谱测定方法。方法采用Thermo C18色谱柱(5μm,4.0 mm×250 mm),流动相为乙腈-0.05 mol/L磷酸二氢钾溶液(50∶50,每100 mL中加十二烷基硫酸钠0.4 g,再以磷酸调节pH值为4.0),检测波长为345 nm,流速1.0 mL/min,柱温30℃。结果盐酸小檗碱的进样量在0.180 34~0.90168μg范围内与峰面积积分值呈良好的线性关系,r=0.999 8。结论本测定方法简便、准确、重复性好,可用于舒通安泰胶囊的质量控制。
|
| 关 键 词： | 舒通安泰胶囊 高效液相色谱法 小檗碱 表小檗碱 黄连碱 巴马汀 含量测定 |
Content Determination of Berberine,Epiberberine, Coptisine and Palmatine in Shutongantai Capsules by HPLC |
| |
| HOU Jian-zhong,YU Li-jian,YANG Cheng. Content Determination of Berberine,Epiberberine, Coptisine and Palmatine in Shutongantai Capsules by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2014, 0(7): 61-63 |
| |
| Authors: | HOU Jian-zhong YU Li-jian YANG Cheng |
| |
| Affiliation: | 1.Jiuquan Institute for Food and Drug Control, Jiuquan 735000, China; 2.Jiuquan Hospital of TCM, Jiuquan 735000, China) |
| |
| Abstract: | Objective To build a method for determination of berberine, epiberberine, coptisine and palmatine in Shutongantai Capsules by HPLC.Methods Thermo C18 column （5μm, 4.0 mm× 250 mm） was used with acetonitrile-0.05 mol/L potassium dihydrogen phosphate solution （50∶50, 0.4 g sodium dodecyl sulfate was added in each 100 mL solution, to adjust the pH 4.0 with phosphoric acid） as mobile phase;the flow rate was 1.0 mL/min;detection wavelength was 345 nm;column temperature was 30℃.Results Berberine hydrochloride showed a good linear relationship in the range of 0.180 34-0.901 68μg （r=0.999 8）.Conclusion The method is simple, accurate and reproducible, and can be used for the quality control of Shutongantai Capsules. |
| |
| Keywords: | Shutongantai Capsules HPLC berberine epiberberine coptisine palmatine content determination |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
