新型CpG ODN增强乙肝疫苗诱导IgG2a类抗体产生的实验研究

目的：寻找能增强乙肝疫苗刺激IgG2a类抗体产生，使机体处于Th1样免疫环境的新型乙肝疫苗佐剂。方法：选用自行设计的A、B、C型CpGODN，并以发表的A、B型CpGODN作为阳性对照，与重组乙肝疫苗混合后于第0．4周免疫BALB／C小鼠，用ELISA法检测免疫小鼠血清中HBsAb水平及种类。结果：各型CpGODN都能增强乙肝疫苗刺激HBsAb的产生水平，CpGODN＋乙肝疫苗免疫的小鼠血清中HBsAb类型为IgG2a〉〉IgG1，而单独应用商品化重组乙型肝炎疫苗的小鼠血清中HBsAb类型为IgG1〉〉IgG2a。结论：各型CpGODN对重组乙型肝炎疫苗含AI（OH）3佐剂]均具有增效作用，而且可以诱导机体产生倾向于Th1途径的免疫应答反应。

Study of New CpG ODNs on enhancement of IgG2a production induced by hepatitis B vaccine

Objective:To search for new adjuvant for hepatitis B vaccine that could stimulate the production of IgG2a and induce Th1-like immune circumstance.Methods:BALB/c mice were immunized with recombinant hepatitis B vaccine only or combined with CpG ODNs designed by our lab(including type A, B and C) and CpG ODNs published in articles at 0th and 4th week. The HBsAb level and the IgG subsets in the serum of mice were measured by ELISA.Results:All types of CpG ODN could enhance the production of HBsAb stimulated by hepatitis B vaccine. The level of IgG subsets of mice immunized with hepatitis B vaccine combined with CpG ODN was IgG2a>>IgG1, and that of mice immunized with hepatitis B vaccine only was IgG1>>IgG2a.Conclusion:All types of CpG ODN can enhance the effect of recombinant hepatitis B vaccine and induce Th1-like immune response.