Specific activation,signalling and secretion profiles of human platelets following PAR-1 and PAR-4 stimulation |
| |
| Authors: | Kim Anh Nguyen Hind Hamzeh-Cognasse Sandrine Laradi Bruno Pozzetto Olivier Garraud |
| |
| Affiliation: | 1. Université de Lyon, Saint Etienne, France,;2. Université de Lyon, Saint Etienne, France,;3. Etablissement Fran?ais du Sang – Auvergne-Loire, Saint-Etienne, France, and;4. INTS – Institut National de la Transfusion Sanguine, Paris, France |
| |
| Abstract: | Blood platelets play a central haemostatic function; however, they also play a role in inflammation and are capable of secreting various cytokines, chemokines and related products. The purpose of this study was to identify subtle variations in platelet physiology using proteomics. We compared the levels of membrane proteins (n?=?3), α and δ granule proteins (n?=?18), and signalling proteins (n?=?30) from unstimulated platelets with those of protease-activated receptor (PAR)-1- and PAR-4-stimulated platelets (n?=?10). The vast majority of these proteins responded similarly to PAR-1 or PAR-4 engagement. However, differences were observed within membrane CD40L expressed, and α granule GRO-α and MDC secreted proteins. |
| |
| Keywords: | Immunologic factors inflammation platelet protease-activated receptors signal transduction |
|
|
