Serum Leptin Levels,Leptin Receptor Gene (LEPR) Polymorphism,and the Risk of Systemic Lupus Erythematosus in Kashmiri Population |
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Authors: | Dil Afroze Adfar Yousuf Raihana Ali Fizalah Kawoosa Tahseena Akhtar Sabeeha Reshi |
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Institution: | 1. Department of Immunology and Molecular Medicine Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Jammu and Kashmir, Indiaafrozedil@gmail.com;3. Department of Immunology and Molecular Medicine Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Jammu and Kashmir, India |
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Abstract: | The study is conducted to evaluate relationship between LEPRQ223R (Gln?>?Arg) polymorphism, serum leptin levels, soluble leptin receptor (SOb-R) levels and SLE risk in Kashmiri population.LEPR genotyping was done by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 100 unrelated SLE patients and equal number of healthy control subjects. Leptin and SOb-R levels were measured by ELISA assays. The present study showed higher frequency of variant genotype (AG?+?GG) in cases compared to controls OR?=?2.52, CI?=?1.18–5.35, p?=?0.03]. Moreover the rare (G) allele was significantly more predominant in cases than controls OR?=?1.49, p?=?0.04]. Interestingly a positive association between the variant genotype and the development of arthritis OR?=?11.8, CI?=?1.6–85.1, p?=?0.002] and an inverse association with cardiac disorder OR?=?0.09, CI?=?0.02–0.46, p?=?0.001] was observed in this study. Furthermore the study showed significant differences of leptin levels in SLE patients and controls (23.9?±?19.5 vs 14.8?±?10.4, p?<?0.001). SLE patients in the highest quartile leptin levels (≥32.5?ng/mL) were significantly more likely to have higher BMI (p?=?0.001) and increased risk of developing arthritis (p?=?0.02). Furthermore positive association was observed between the variant genotype(AG?+?GG) and leptin levels (p?=?0.001) in SLE patients. Thus, it is evident from our study that LEPRQ223R polymorphism and elevated leptin levels are associated with increased susceptibility of SLE in Kashmiri population. |
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Keywords: | Genotyping Kashmir valley leptin leptin receptor North India polymerase chain reaction systemic lupus erythematosus |
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