祛疣洗剂治疗尖锐湿疣的临床研究及其作用机制

目的观察祛疣洗剂治疗尖锐湿疣的临床疗效，探讨其可能的作用机制。方法分别用祛疣洗剂及微波治疗尖锐湿疣患者各30例。用免疫组化SABC法检测祛疣洗剂治疗前后基底细胞和棘细胞的Ki-67表达及表皮bax、bcl-2的表达，同时采用TUNEL法检测治疗前后表皮细胞的凋亡情况。结果治疗组治愈率及有效率分别为：53.33％、93.33％，对照组治愈率及有效率分别为：47．67％、80．00％，差异无显著性（P〉0．05）；两组复发率分别为6．25％、42．86％，差异有显著性（P=0．031）。祛疣洗剂治疗后基底细胞和棘细胞增殖指数明显低于治疗前（P〈0．05），而bax、bcl-2在祛疣洗剂治疗前后表皮的表达没有明显差异（P〉0．05），TUNEL检测的细胞凋亡指数在治疗后略高于治疗前，但差异无显著性（P〉0．05）。结论祛疣洗剂治疗尖锐湿疣的作用机制，可能是通过抑制表皮的基底细胞和棘细胞增殖而发挥作用的。

Efficacy of Qu-You Lotion on Treatment of Condyloma Acuminatum and Its Possible Mechanism

Objective To observe the effects of Qu-You lotion for condyloma acuminatum （CA） and to explore its mechanism. Methods 60 patiants with CA were randomly divided into two groups： 30 cases in treatment group were treated with Qu-You lotion and 30 cases in control group were treated with micro wave. Expression of Ki-67 in basal cells and acanthocytes, bax and bcl-2 expression of epidermic cells were detected by SABC immunohistochemicel technique. Cell apoptosis index was determinated by TUNEL method. Results The clinical cure rate and response rate in the treatment group was 53.33% and 93.33% respectively. The clinical cure rate and response rate in the control group was 47.67% and 80.00% respectively. There was no significant difference between them. The relapse rate in the treatment group was significantly lower than that in control group （6.25%and 42.86%）.The cell proliferating index labeling in Ki-67 of basal cells and acanthocytes after the treatment were lower than that of before （P〈0.05）. No differences of bax and bcl-2 expression were observed hefore and after the treatment （P〉0.05）. Cell apoptosis index labeling in TUNEL after the treatment was slightly higher than that of before（P〉0.05）. Conclusion It is indicate that Qu-You lotion can improve the cure rate and reduce the relapse rate of CA. Its effective mechanism may take effect through inhibiting cell proliferating of basal cells and acanthocytes of epidermis.