2007—2008年河北省急性迟缓性麻痹病例脊灰病毒核苷酸变异情况分析

目的及时发现河北省可能出现的脊灰疫苗衍生病例（VDPV）。方法对河北省2007—2008年急性弛缓性麻痹（AFP）病例的粪便标本进行病毒分离和血清定型。所有标本用L20B、RD细胞同时进行病毒分离，用PCR—RFLP法做型内鉴定。结果从报告的763例AFP病例粪便标本中分离到42株脊髓灰质炎病毒株（PV），其中Ⅰ型4株，Ⅱ型11株，Ⅲ型11株，混合型13株，PV＋NEPV共3株；将混合株进行单型分离后，共得到58株，以Ⅲ型PV为主，均为疫苗变异株；发病年龄在1岁以内儿童占84．48％，男性发生变异39株，女性发生19株。结论河北省2007—2008年分离到的脊髓灰质炎疫苗病毒有部分变异，未发现疫苗衍生脊灰病毒。

Analysis on Nucleotide Variation of Poliovirus Isolated From Acute Flaccid Paralysis Cases in Hebei Province, 2007 -2008

Objective To detect vaccine derived polioviruses （VDPVs） timely in Hebei province and to keep the achievement in polio eradication. Methods The polioviruses were isolated from stool samples of acute flaccid paralysis（AFP） cases from 2007 to 2008 by using L20B and RD cells, and identified according to Polio Laboratory Manual. PCR-RFLP was applied in intratypic differentiation. Results Forty two strains of poliovirus （PV） were isolated from 763 AFP cases, including 4 strains of PV type Ⅰ, Ⅱ strains of PV type Ⅱ, 11 strains of PV type Ⅲ, 13 strains of mixed PV, 3 strains of PV ＋ NEPV. After single type isolation of mixed strains, 58 strains of PV were obtained, most of them were PV type Ⅲ which were vaccine variant strains. The cases of children less than 1 year old accounted for 84. 48%. Thirty nine variant strains were found in males, 19 were found in females. Conclusion VDPVs were not found in Hebei province from 2007 to 2008, it is necessary to improve vaccination to maintain high level of coverage, strengthen high quality AFP surveillance and keep polio-free status. Key words Acute flaccid paralysis; Poliovirus, Nucleotides; Vaccine-derived polio viruses.