都柏林念珠菌和白念珠菌粘附能力的比较研究

目的：比较研究都柏林念珠菌（candida dubliniensis,CD)和白念珠菌（candida albicans,CA)的粘附能力。及4%小苏打液和0.2%洗泌肽对CD和CA粘附力的影响。方法：检测糜烂型口腔扁平苔藓（oral lichen planue,OLP)，非糜烂型OLP和健康对照组3种不同来源颊粘膜上皮细胞（buccal epithelial cells,BEC)。经0.2%洗泌汰和4%小苏打液体外处理前后，BEC上粘附孢子数。结果：（1）处理前，3种不同来源BEC上，粘附CD孢子数显著高于粘附CA的孢子数。（2）糜烂型OLP和非糜烂型OLP来源的BEC上，粘附的孢子数显著高于健康对照组；（3）3种不同来源BEC经0.2%洗泌汰和4%小苏打液处理后，其上粘附的孢子数显著减少。结论：（1）CD对BEC粘附力比CA强；（2）4%小苏打液能降低CD和CA对BEC的粘附，4%小苏打液和0.2%洗泌汰在降低念珠菌对BEC的粘附上，具有相近的作用。

Comparative research on the adhesion of Candida Dubliniensis to Human Buccal Epithelial with That of Candida Albicans in Vitro

Objective: To research the effect of Sodium Bicarbonate and Chlorhexidine on the adhesion of candida dubliniensis (CD) and candida albicans (CA) to human buccal epithelial cells (BEC). Methods: The suspension of BEC from 20 cases oral lichen planus(OLP) and 10 health donors and CD or CA were prepared by common methods, and then the blastopores of CD or CA attached to BEC (exposed or no-exposed 4% Sodium Bicarbonate and 0 2% Chlorhexidine) were counted microscopically. Results:(1) The blastopores of CD or CA attached to BEC in control group significantly decreased compared with erosive OLP and non-erosive OLP(P<0.01); (2) In each group, after BEC exposed to 4% Sodium Bicarbonate and 0 2% Chlorhexidine, the blastopores of CD significantly increased compared with that of CA. (3) In each group, after BEC exposed to 4% Sodium Bicarbonate and 0 2% Chlorhexidine, the blastopores both CD and CA significantly decreased compared with that of before. Conclusion: (1) Adhesive ability of CD to BEC was significantly higher than that of CA. (2) 4% Sodium Bicarbonate could reduce the adhesion of CD and CA to BEC and could be substituted by 0 2% Chlorhexidine clinically.