粉防己碱对激素非依赖性人前列腺癌细胞株PC-3增殖和凋亡影响的研究

目的 探讨粉防己碱(TET)对激素非依赖性人前列腺癌细胞株PC-3细胞增殖抑制和凋亡作用及其机制。方法 应用CCK法观察不同浓度TET（1、2、4、8 μg/mL）对人前列腺癌细胞株PC-3生长的抑制作用；用Annexin-V与PI双染法流式细胞术分析TET对PC-3细胞凋亡的影响；RT-PCR检测TET对人前列腺癌细胞株PC-3 IL-8表达水平的影响。结果 TET对前列腺癌PC-3细胞株增殖有抑制作用，其抑制效应呈剂量依赖性。不同浓度TET作用48 h后，前列腺癌PC-3细胞株的凋亡率随着浓度的增高而升高，差异具有统计学意义（P<0.01）。不同浓度TET作用48 h后IL-8 mRNA表达水平随着浓度的增加而降低，差异具有统计学意义（P<0.01）。结论 TET能抑制人前列腺癌细胞株PC-3细胞增殖，并促进人前列腺癌细胞株PC-3的细胞凋亡，其作用机制可能与其下调IL-8 mRNA表达有关。

The effect of tetrandrine on proliferation and apoptosis of human androgen refractory prostate cancer PC-3 cell line

Objective  To investigate the mechanism of proliferation and apoptosis of human androgen-refractory prostate cancer PC-3 cell line by tetrandrine. Methods  The effects of TET（1 μg/mL，2 μg/ml，4 μg/mL，8 μg/mL）on the growth of PC-3 cell line were measured by CCK assay. The apoptosis of cell line induced by TET（1 μg/mL，2 μg/mL，4 μg/mL，8 μg/mL） was evaluated by Annexin-V and PI followed by flow cytometry （FCM）. The effects of TET on the expression of IL-8 were measured by RT-PCR.Results   TET inhibited the proliferation of PC-3 cell line in a dose dependent manner. After treated with TET（1 μg/mL，2 μg/mL，4 μg/mL，8 μg/mL）for 48 h, the apoptotic rate increased with the increase of drug concentration（P<0.01）.Similarly，the expression of IL-8 mRNA decreased gradually with the increase of drug concentration（P<0.01）.Conclusions  TET can inhibt the proliferation of PC-3 cell line，and induce apoptosis. This may be associated with the decreasing expression of IL-8 mRNA.