多种中药对泛耐药肺炎克雷伯菌体外抗菌活性筛选

目的筛选对泛耐药肺炎克雷伯菌有抗菌活性的中药，并初步探究其体外抑菌机制。方法从60种中药颗粒剂中，筛选对28株泛耐药菌有体外抗菌活性的中药。调整有抗菌活性药液pH至中性，比较调整pH前后抑菌效果差异。检测有抑菌活性中药的最小抑菌浓度（MIC）、最小杀菌浓度（MBC）、联合抑菌效果以及对细菌细胞外碱性磷酸酶（ALP）活性改变。结果筛选出黄芩、黄连、麻黄和鸡血藤等4种对泛耐药菌均有抑菌作用的中药，其MIC依次为:64、64（82.1%黄连菌株）、128（19.9%）、128、256 mg/mL；MBC依次为:128、64（28.6%黄连菌株）、128（71.4%）、256、512 mg/mL。4种中药调整pH前后抑菌效果均无差异（P>0.05）。黄连和黄芩联合应用时的FIC值均>2。随着4种药物浓度的增加，ALP的浓度均升高，其中黄芩和黄连作用后的ALP浓度升高更为明显。结论黄芩、黄连、麻黄和鸡血藤具有良好的体外抗菌效果，其中黄芩和黄连的抗菌机制可能与增加细胞膜的通透性有关；但黄芩和黄连联合用药有拮抗作用。

Screening of the traditional Chinese medicine with antibacterial activity against pan-drug resistant Klebsiella pneumoniae in vitro

ObjectiveTo screen the traditional Chinese medicine with antibacterial activity against pan-drug resistant Klebsiella pneumoniae, and explore its antibacterial mechanism in vitro.MethodsAmong 60 traditional Chinese medicine granules, Chinese medicines with antibacterial activity against 28 pan-resistail strains were screened.The pH value of the antibacterial active liquid was adjusted to neutral, and the antibacterial effects were compared between before and after adjusting pH value.The minimum inhibitory concentration(MIC), minimum bactericidal concentration(MBC), combined bacteriostatic effects and changes of extracellular alkaline phosphatase(ALP) activity of these Chinese medicines with antibacterial activity were detected.ResultsFour kinds of traditional Chinese medicine with antibacterial activity against pan-resistant bacteria were screened out, which included the Scutellariae Radix, Coptis Rhizoma, Ephedrae Herba and Spatholobi Coulis.The MIC of the Scutellariae Radix, Coptis Rhizoma, Ephedrae Herba and Spatholobi Caulis was 64 mg/mL, 64 mg/mL, 128 mg/mL, 128 mg/mL and 256 mg/mL, respectively.The MBC of the Scutellariae Radix, Coptis Rhizoma, Ephedrae Herba and Spatholobi Caulis was 128 mg/mL, 64 mg/mL, 128 mg/mL, 256 mg/mL and 512 mg/mL, respectively.The differences of the antibacterial effects in four kinds of traditional Chinese medicine between before and after adjusting pH value were not statistically significant(P>0.05).The FIC values of the combined application of Scutellariae Radix and Coptis Rhizoma were more than 2.With the increasing of the concentrations of four drugs, the concentrations of ALP increased, and the increasing degree of ALP concentration was more obvious after Scutellariae Radix and Coptis Rhizoma treated.ConclusionScutellariae Radix, Coptis Rhizoma, Ephedrae Herba and Spatholobi Caulis have good antibacterial effect in vitro, the antibacterial mechanism of Scutellariae Radix and Coptis Rhizoma may be related to the increasing of membrane permeability, but the combination of Ephedrae Herba and Spatholobi Caulis has antagonistic effect.