ADAM17 siRNA和阿霉素联用对前列腺癌PC-3细胞增殖和凋亡的作用

目的 探讨ADAM17 siRNA联合化疗药物阿霉素（ADM）对人前列腺癌细胞株PC-3增殖和凋亡的影响。方法 用ADM处理 ADAM17 siRNA表达质粒稳定转染的PC-3细胞，采用噻唑蓝（MTT）比色法观察细胞增殖率，吖啶橙染色和流式细胞仪（FCM）检测细胞凋亡率，并对凋亡蛋白caspase-3的活性进行对比分析。结果 ADAM17 siRNA和ADM单独及联合应用均能不同程度的降低PC-3细胞增殖率，上调凋亡率。FCM检测数据显示ADAM17 siRNA和ADM单独应用的细胞凋亡率分别为（6.2±1.4）%和（17.2±1.9）%，与ADAM17 siRNA和ADM联合应用的（36.4±3.7）%相比较，差异有显著统计学意义（P<0.01），两者在诱导caspase-3活性方面也具有良好的协同增敏作用。结论 ADAM17 siRNA和ADM对人前列腺癌PC-3均有抑制细胞增殖和促进凋亡的效果，但两者联合应用效果更好。提示分子靶向用药的基因治疗和化疗联合治疗可能是提高疗效，减少前列腺癌术后复发转移风险的重要方法。

Effects of ADAM17 siRNA and doxorubicin on proliferation and apoptosis of prostate cancer PC-3 cells

Objective    To investigate the effects of ADAM17 siRNA combined with adriamycin (ADM) on proliferation and apoptosis of human prostate cancer cell line PC-3.Methods   PC-3 cells stably transfected with ADAM17 siRNA expression plasmid were treated with ADM. The proliferation rate was observed by MTT colorimetry, the apoptosis rate was detected by acridine orange staining and flow cytometry (FCM), and the activity of apoptotic protein caspase-3 was analyzed. Results   Both ADAM17 siRNA and ADM alone and in combination could decrease the proliferation rate and increase the apoptosis rate of PC-3 cells in varying degrees. FCM data showed that the apoptosis rates of ADAM17 siRNA and ADM alone were（6.2±1.4）% and （17.2±1.9）% respectively, which were significantly different from （36.4±3.7）% of ADAM17 siRNA and ADM alone (P<0.01). Both of them also had a good synergistic effect on inducing caspase-3 activity. Conclusions   ADAM17 siRNA and ADM have different effects on the proliferation and apoptosis of human prostate cancer PC-3, but the combination of ADAM17 siRNA and ADM is more effective. It is suggested that gene therapy combined with chemotherapy may be an important method to improve the curative effect and reduce the risk of recurrence and metastasis of prostate cancer.