LPS对A549和Hela细胞SPLUNC1蛋白表达的影响

 目的 探讨脂多糖(LPS)对人肺腺癌上皮A549细胞和人子宫颈癌上皮Hela细胞短的上腭、肺及鼻咽上皮克隆1(short palate, lung and nasal epithelium clone 1, SPLUNC1)蛋白表达的影响。方法 将两类细胞各随机分为对照组、实验组,CCK-8法观察LPS与细胞增殖活性的时间-反应和剂量-反应关系,筛选各自的最佳效应浓度和作用时间,以LPS最佳效应浓度和作用时间处理后,免疫组化和免疫荧光定量法检测两类细胞SPLUNC1蛋白相对表达量的变化。结果 LPS对A549细胞的最佳效应浓度为20 mg/L,最佳效应时间为12 h,对Hela细胞的最佳效应浓度为40 mg/L,12~24 h为其最佳效应时间窗。SPLUNC1在两类细胞中均呈阳性表达,LPS处理后表达均显著上调(P＜0.05),两类细胞间比较,LPS处理后Hela细胞SPLUNC1相对表达量高于A549细胞(P＜0.05),较对照的增量值(Δ)也明显大于A549细胞(P＜0.05)。结论 SPLUNC1在宫颈癌细胞中存在表达,可能是肺腺癌和宫颈癌病变中的保护性因子,具有潜在的临床应用价值。

The influence of LPS on the expression of SPLUNC1 in Hela cells and A549 cells

Objective To explore the influence of LPS on SPLUNC1 in Hela cells and A549 cells.Methods The two cell lines were cultured and divided into the control group and experimental group randomly. CCK-8 was used to study the time-response and dose-response relationship between LPS and proliferation and to screen the optimal concentration and action time of LPS in each cell line. After treatment with the optimal concentration and action time of LPS, the relative expression of SPLUNC1 was measured by immunohistochemical staining and immunofluorescence assay.Results The optimal concentration of LPS for A549 cells was 20 mg/L, and the optimal action time was 12 h. For Hela cells, the values were 40 mg/l and 12-24 h respectively. Both A549 cells and Hela cells expressed SPLUNC1. After LPS exposure,the expression of SPLUNC1 was up-regulated significantly(P＜0.05).Comparison between the two cell lines showed that after treatment with LPS the relative expression of SPLUNC1 in Hela cells was significantly higher than in A549 cells (P＜0.05), and the increment(Δ) of SPLUNC1 compared to the control in Hela cells was also significantly greater than in A549 cells(P＜0.05).Conclusions SPLUNC1 is expressed in cervical cancer. It can be a protective cell agent against lung adenocarcinoma and cervical cancer with potential clinical value.