非小细胞肺癌中p-mTOR、Beclin1及Atg1蛋白表达及其临床意义

目的检测自噬相关蛋白p-mTOR、Beclin1及Atg1在非小细胞肺癌及正常肺组织中表达水平，探讨细胞自噬在肺癌演变过程中的作用机制。 方法应用免疫组织化学法测定96例非小细胞肺癌、64例正常肺泡、60例正常支气管断端组织p-mTOR、Beclin1及Atg1蛋白表达水平，分析其表达差异与临床病理特征相关性。 结果①Beclin1、Atg1蛋白在非小细胞肺癌组织中表达显著下降，且二者表达水平正性相关，p-mTOR蛋白表达显著升高；②随肿瘤增大、分化程度下降及出现淋巴结转移，Beclin1蛋白表达显著下降；③随肿瘤增大及出现淋巴结转移，Atg1蛋白表达显著下降；④随肿瘤分化程度下降及出现淋巴结转移，p-mTOR蛋白表达显著升高；⑤p-mTOR与Beclin1及与Atg1表达均呈负性相关。 结论自噬在非小细胞肺癌发病中起重要作用，自噬相关蛋白Beclin1、Atg1起协同抑癌作用，p-mTOR蛋白促进非小细胞肺癌发生、发展。

Expression and clinical significance of p-mTOR,Beclin1 and Atg1 proteins in non-small cell lung cancer

ObjectiveThe expression levels of autophagy related proteins p-mTOR, Beclin1 and Atg1 in non-small cell lung cancer and normal lung tissues were detected to explore the mechanism of autophagy in the evolution of lung cancer. MethodsImmunohistochemistry was used to determine the expression levels of p-mTOR, Beclin1 and Atg1 protein in 96 cases of non-small cell lung cancer, 64 cases of normal alveolar tissue and 60 cases of normal bronchial duodenal tissue, and to analyze the correlation between the expression differences and clinic pathological features. Results①Beclin1 and Atg1 proteins were significantly decreased in non-small cell lung cancer tissues and their expression levels were positively correlated. P-mTOR protein expression was significantly increased. ②Beclin1 protein expression decreased significantly with tumor enlargement, differentiation and lymph node metastasis. ③Atg1 protein expression decreased significantly with tumor enlargement and lymph node metastasis. ④The expression of p-mTOR protein increased significantly with the decrease of tumor differentiation and lymph node metastasis. ⑤p-mTOR was negatively correlated with Beclin1 and Atg1 expression. ConclusionAutophagy plays an important role in the pathogenesis of NSCLC. Beclin1 and Atg1, autophagy related proteins, play a synergistic role in tumor inhibition, and p-mTOR protein promotes the occurrence and development of NSCLC.