冷沉淀制备时间对新鲜冰冻血浆凝血因子及纤维蛋白原含量的影响

目的探讨冷沉淀制备时间对新鲜冰冻血浆凝血因子及纤维蛋白原含量的影响。方法选择2018年10月-2019年4月期间制备的新鲜冰冻血浆160份,根据采集后制备成新鲜冰冻血浆时间分为4组,分别为6 h组、8 h组、12 h组和18 h组,每组各40份。将4组新鲜冰冻血浆制备成冷沉淀,对比FⅧ、Fg含量和冷沉淀中凝血因子质量。结果随制备时间延长,FⅧ含量逐渐降低,差异有统计学意义(P<0.05);不同时间段制备的冷沉淀中Fg含量对比,差异无统计学意义(P>0.05);6 h组合格率为97.50%高于8 h组87.50%、12 h组75.00%和18 h组55.00%,差异有统计学意义(P<0.05),且6 h组、8 h组和12 h组均达到标准,18 h组未达到标准。结论新鲜冰冻血浆的制备时间对冷沉淀中的凝血因子影响较大,对纤维蛋白原影响无明显影响,并且全血制备新鲜冰冻血浆的最佳时间在6~8 h,最长不能超过12 h。

Effect of preparation time of cold precipitation on contents of blood coagulation factors and fibrinogen in fresh frozen plasma

Objective To investigate the effect of the preparation time of cold precipitation on the contents of blood coagulation factors and fibrinogen in fresh frozen plasma. Methods A total of 160 fresh frozen plasma samples were prepared from October 2018 to April 2019. According to the time of collection, the fresh frozen plasma was divided into 4 groups: 6-h group, 8-h group, 12-h group, and 18-h group, with 40 samples in each group. The fresh frozen plasma of four groups was prepared into cold precipitation, and the contents of FVIII and Fg, and the quality of coagulation factors in the cold precipitation were compared among the four groups. Results With the prolongation of preparation time, the content of FVIII decreased gradually, and the difference was statistically significant (P<0.05); the Fg contents in the cold precipitation prepared in different time periods were not statistically significantly different (P>0.05); the qualified rate of 6-h group was 97.50%, which was higher than that of the 8-h group (92.50%), 12-h group (80.00%), and the 18-h group (55.00%) (P<0.05). Conclusion The preparation time of fresh frozen plasma has great effect on coagulation factors in cold precipitation but no significant effect on fibrinogen, and the optimal time for whole blood preparation of fresh frozen plasma was 6-8 hours, and the maximum time should be less than 12 h.