莪术醇对肝星状细胞Rho-ROCK信号通路作用的实验研究

目的:研究莪术醇对肝星状细胞Rho-ROCK信号通路的作用,以此来分析莪术醇抗肝纤维化的作用机制。方法:用莪术醇孵育HSC-T6细胞48 h后用MTT法检测细胞增殖率,用免疫印迹和RT-PCR检测Rho-ROCK信号通路上关键分子RhoA和ROCK2的表达和含量。结果:RT-PCR检测发现莪术醇高、中、低浓度对Rho-ROCK信号通路中的关键分子RhoA和ROCK2mRNA的表达较模型组有抑制作用,而且这种抑制作用有明显的剂量依赖性,差异有显著性(P<0.05);WB检测发现莪术醇高、中、低浓度对Rho-ROCK信号通路中的关键分子RhoA和ROCK2的表达较模型组有抑制作用,而且这种抑制作用有明显的剂量依赖性,差异有显著性(P<0.05)。结论:莪术醇可以抑制Rho-ROCK信号通路的活动,达到抗肝纤维化的效果。

Effect of curcumol on Rho-ROCK signaling pathway in hepatic stellate cells

OBJECTIVE To study the effect of curcumol on Rho-ROCK signaling pathway in hepatic stellate cells, and to analyze the mechanism of curcumol on hepatic fibrosis. METHODS HSC-T6 cells were incubated with curcumol for 48 hours. The cell proliferation rate was detected by MTT assay. The expression and content of RhoA and ROCK2 in Rho-ROCK signaling pathway were detected by Western blot and RT-PCR. RESULTS Results of RT-PCR showed that the expression of RhoA and ROCK2 mRNA in Rho-ROCK signaling pathway was inhibited by high, medium and low concentrations of curcumol in a dose-dependent manner compared with the model group, and the difference was statistically significant (P<0.05). Wb assay showed that The expression of RhoA and ROCK2 in Rho-ROCK signaling pathway was inhibited by high, medium and low concentrations of curcumol in a dose-dependent manner compared with the model group, and the difference was statistically significant (P<0.05). CONCLUSION Curcumol can inhibit the activity of Rho-ROCK signaling pathway and achieve the effect of anti-hepatic fibrosis.