卡莫氟对人子宫颈癌细胞系HeLa增殖及侵袭的影响及机制

目的 探讨卡莫氟（carmofur）对人子宫颈癌细胞增殖、侵袭的影响及其机制。 方法 将卡莫氟配置成不同浓度（0.4、0.8和1.2 mg/L）作用宫颈癌HeLa细胞，并设置对照组。使用细胞计数试剂盒8（CCK-8）法检测细胞情况，用Transwell实验检测细胞侵袭情况，通过Western blotting和Real-time PCR法检测基质金属蛋白酶7（MMP-7）、β-连环蛋白（β-catenin）和连环蛋白P120（P120 ctn）和mRNA的表达水平。 结果 与对照组相比，药物处理组随卡莫氟浓度的上升，细胞增殖能力降低，卡莫氟作用24 h即可抑制细胞增殖，且随着作用时间的延长，细胞增殖速率显著降低（均P<0.05）。与对照组相比，卡莫氟组可抑制HeLa细胞的侵袭（P<0.05）。与对照组相比，卡莫氟组可降低MMP-7、β-catenin和P120ctn蛋白水平和mRNA水平（P<0.05）。结论 卡莫氟能抑制HeLa细胞的增殖、侵袭，其机制可能与下调MMP-7、β-catenin和P120ctn表达水平有关。

Effect of carmofur on proliferation and invasion of cervical cancer cell line HeLa and its mechanism

Objective To investigate the effect of carmofur on the proliferation and invasion of cervical cancer cell line HeLa and to study its mechanism. Methods Carmofur was prepared into different concentrations (0.4 mg/L, 0.8 mg/L, and 1.2 mg/L) and the control group was set. Cell counting kit-8(CCK-8) method was used to detect the viability of the cells. Transwell technique was used to detect cell invasion. The expression levels of matrix metalloproteinase(MMP)-7, β-catenin and P120 catenin(P120 ctn) and mRNA were detected by Western blotting and Real-time PCR. Results Compared with the control group, the cell proliferation ability decreased with the increase of the concentration of carmofur in the drug treatment group, and the cell proliferation was inhibited after 24 hours of carmofur exposure, and the cell proliferation rate decreased significantly with the prolongation of the action time (all P<0.05). Compared with the control group,carmofur group inhibited the invasion of HeLa cells (P<0.05). Compared with the control group, the carmofur group decreased MMP-7, β-catenin and P120 catenin levels and mRNA levels (P<0.05). Conclusion HCFU can inhibit the proliferation of HeLa cells, and its mechanism might be related to the down regulation of MMP-7, β-catenin and P120 catenin expression levels.