甲基苯丙胺不同给药时程及天麻素的干预对建立大鼠条件性位置偏爱模型的影响

目的 比较甲基苯丙胺不同给药时程对建立大鼠条件性位置偏爱 (CPP) 模型的影响及天麻素对甲基苯丙胺CPP模型的干预作用。方法 采用甲基苯丙胺10 mg/kg腹腔注射 (ip) 10 d, 确认动物产生CPP效应后, 再分别注射7 d、14 d、28 d建立3组不同依赖时程的大鼠甲基苯丙胺依赖模型;采用天麻素10 mg/kg腹腔注射28 d进行干预, 随后再进行CPP检测, 测定天麻素给药对大鼠在伴药箱停留时间的影响。结果 与对照组比较, 甲基苯丙胺给药7 d、14 d、28 d 3组大鼠在伴药箱停留时间均明显延长 (P<0.05) ;14 d组与7 d组、28 d组比较, 差异有统计学意义 (P<0.05) , 7 d组与28 d组比较, 差异无统计学意义 (P> 0.05) ;甲基苯丙胺+天麻素组与甲基苯丙胺组比较, 差异有统计学意义 (P<0.05) , 且天麻素干预组在伴药箱停留时间明显缩短。结论用甲基苯丙胺10 mg/kg腹腔注射给药, 7 d组、14 d组、28 d组均可诱导大鼠CPP效应的形成且给药14 d的大鼠CPP模型行为学改变较显著;天麻素干预具有缓解大鼠CPP效应的作用。

Establishment of the Methamphetamine Induced Rat Conditioned Place Preference Model and the Intervention Effect of Gastrodin

Objective To establish the methamphetamine induced rat conditioned place preference (CPP model, compare the behaviour effects of different time courses of methamphetamine administration and the intervention effect of gastrodin on rat. Methods The rats were intraperitoneally injected (ip) with 10 mg/kg of methamphetamine for 10 days;after confirming the CPP effects were established in rats, the animals of experimental groups were injected with 10 mg/kg of methamphetamine for 7 days, 14 days, and 28 days respectively, to establish three methamphetamine dependent rat model with different dependent times. And then, the gastrodin intervention groups were given 10 mg/kg of gastrodin for 28 days. Then, CPP test were conducted. Results Compared with the control group, the times spent in the drug-paired chamber were increasd significantly in groups of MA-dependent 7 days, 14 days, and 28 days. Moreover, there was statistical difference between 14 days group and 7 days group or 28 days group, but there was no statistical difference between 7 days group and 28 days group.There was significant difference between the MA+Gastrodin groups and the MA groups, and gastrodin intervention groups had shorter time in the drug-paired chamber. Conclusions Constant injection of methamphetamine for 7 days, l4 days, and 28 days can successfully induce CPP effects and establish MA dependent rat model. The CPP effect of methamphetamine 14 days group is more significant than other groups. Gastrodin inervention can alleviate the CPP effect of rat.