双肝移植后移植物萎缩的动物实验探究

目的通过动物实验验证临床双肝移植后移植物萎缩的现象。 方法清洁级雄性SD大鼠18只，8～10周龄，体质量230～250 g，术前禁食12 h。12只SD大鼠作为供体，6只作为受体。建立大鼠双肝移植模型，通过磁共振检查观测受体大鼠术后移植物体积变化。采用成组t检验比较受体大鼠左、右侧移植物体积和重量。P<0.05为差异有统计学意义。 结果移植肝叶均为双右上叶。供肝重量和受体大鼠肝重量分别为(4.30±0.06)和(9.4±0.2)g，移植物与受体大鼠体质量、肝重量比分别为1.79%～1.88%、45.7%～46.8%。手术时间(70±4)min，冷缺血时间(30.0±1.5)min，热缺血时间(12.0±1.5)min，无肝期(20.0±2.5)min。6只受体大鼠双肝移植后1 d行磁共振检查，两侧移植物体积相同；术后15 d行磁共振检查，有3只发生单侧移植物萎缩。 结论大鼠双肝移植后部分受体一侧移植物会发生萎缩。

Animal experiments study on graft atrophy after dual-grafts liver transplantation

ObjectiveTo verify the phenomenon of graft atrophy after clinical dual-graft liver transplantation through animal experiments. MethodsEighteen clean male SD rats, aged 8-10 weeks, weighing 230-250 g, were fasted for 12 hours before surgery. Twelve SD rats served as donors and 6 served as recipients. A rat dual-graft liver transplantation model was established, and the volume changes of the recipient rats after transplantation were observed by magnetic resonance technology. The volume and mass of left and right grafts of donor liver were compared by group t test. P<0.05 was considered statistically significant. ResultsThe liver lobes were double right superior lobe. The average liver mass of the donor rats was (4.30±0.06) g, and the average total liver mass of the recipient rats was (9.4±0.2) g. The ratio of graft to body weight of recipient rats was 1.79% to 1.88%, and the ratio of graft to liver mass of recipients was 45.7% to 46.8%. The operation time was (70±4 ) min, the cold ischemia time was (30.0±1.5) min, the warm ischemia time was (12.0±1.5) min, and the anhepatic phase was (20.0±2.5) min. Six recipient rats underwent MRI at 1 day after double liver transplantation, and the volume of the grafts on both sides was the same. After 15 days after MRI, 3 rats suffered unilateral graft atrophy. ConclusionsPartial recipient-side grafts atrophy after dual-graft liver transplantation in rats.