基于斑马鱼模型三棱抗血栓活性部位筛选及其化学成分研究

目的:筛选中药三棱抗血栓活性部位并对其化学成分进行研究。方法:采用斑马鱼血栓模型对中药三棱80%乙醇提取物的石油醚部位、乙酸乙酯部位、正丁醇部位和水部位的抗血栓作用进行测试。以硅胶柱色谱等方法对抗血栓作用显著的部位进行分离纯化,通过NMR, MS等方法并结合文献数据鉴定化合物结构。结果:乙酸乙酯萃取物能显著增加斑马鱼血栓模型的心脏染色强度(P<0. 01),其余各部位抗血栓作用不明显。在该部位分离得到11个化合物,分别鉴定为对羟基苯甲酸(1)、5-羟甲基糠醛(2)、对甲氧基苯甲醛(3)、对羟基苯甲醛(4)、对香豆酸(5)、阿魏酸(6)、香草酸(7)、原儿茶酸(8)、香兰素(9)、Methyl-3, 6-dihydroxy-2-[2-(2-hydroxyphenyl)-ethynyl]benzoate(10)及1, 3-O-二阿魏酰基甘油(11)。结论:三棱提取物的乙酸乙酯萃取部位具有显著的抗血栓作用,其主要化学成分为酚酸类化合物。

Screening of antithrombotic active Fractions and chemical constituents in Sparganii Rhizoma based on zebrafish model

OBJECTIVE To screen antithrombotic active fractions and investigate chemical constituents of Sparganii Rhizoma. METHODS Zebrafish model was used to test thepetroleum ether, ethylacetate, butanol and water dissolving fractions of 80% ethanol extract of,Sparganii Rhizoma. The compounds of active fractions were isolated and purified by silica gel column chromatography, and their structures were identified by NMR and MS combined with literature data. RESULTS Ethylacetate extract significantly increased heart staining intensity in zebrafish thrombosis models (P<0.01), other parts weren't obvious. 11 compounds were isolated at this site and identified as hydroxybenzoic acid(1), 5-hydroxymethylfurfural(2), p-methoxybenzaldehyde(3), p-hydroxybenzaldehyde(4), coumaric acid(5), ferulic acid(6), vanillic acid(7), protocatechuic acid(8), vanillin(9), methyl-3, 6-dihydroxy- -2-[2-(2-hydroxyphenyl)-ethynyl]benzoate(10) and 1, 3-O-diferheylglycerol(11).CONCLUSION The ethyl acetate extract of the Sparganii Rhizoma has a significant antithrombotic effect, and its main chemical component are phenolic acid compounds.