血清中8-OHdG、MDA、SOD和T-AOC对脑卒中患者的诊断价值

目的分析血清中8-OHdG、MDA、SOD和T-AOC对脑卒中患者的诊断价值。 方法采用酶联免疫吸附试验(ELISA)测定缺血性脑卒中(IS)102例、出血性脑卒中(ICH)98例和正常对照组95例血清8-羟基脱氧鸟苷酸(8-OHdG)、丙二醛(MDA)、超氧化物歧化酶(SOD)，采用菲啉类物质比色法检测三组总抗氧化能力(T-AOC)。用ROC曲线下面积分析8-OHdG、MDA、SOD和T-AOC在脑卒中的诊断价值。 结果IS和ICH组血清8-OHdG和MDA水平均高于正常对照组(P<0.05)，SOD和T-AOC水平均低于正常对照组(P<0.05)。IS组血清8-OHdG、SOD和T-AOC水平高于ICH组(P<0.05)，而两组之间MDA水平无统计学差异(P>0.05)；8-OHdG与MDA、SOD和T-AOC水平呈正相关(P<0.01)，MDA与SOD无相关性(P>0.05)，MDA与T-AOC呈正相关(P<0.05)。8-OHdG、MDA、SOD和T-AOC诊断IS与健康者的特异性分别为84%，82%，89%，91%。8-OHdG、MDA、SOD和T-AOC诊断ICH与健康者的特异性分别为71%，84%，79%，72%。8-OHdG和T-AOC与脑卒中密切相关且是脑卒中的保护性因素；MDA和SOD与脑卒中密切相关且是脑卒中的危险因素。 结论血清8-OHdG、MDA、SOD和T-AOC是脑卒中患者发生氧化应激的灵敏指标。8-OHdG、SOD和T-AOC水平与脑卒中病情有明显相关度，对脑卒中患者的鉴别诊断具有较高实用价值。

Diagnostic value of serum 8-OHdG,MDA, SOD and T-AOC in patients with stroke

ObjectiveTo analyze the diagnostic value of 8-OHdG, MDA, SOD and T-AOC in patients with stroke. MethodsThe levels of serum 8-hydroxydeoxyguanosine monophosphate (8-OHdG), malondialdehyde (MDA) and superoxide dismutase (SOD) in 102 cases of ischemic stroke (IS group), 98 cases of hemorrhagic stroke (ICH group) and 95 healthy subjects (the normal control group), were detected by enzyme-linked immunosorbent assay (ELISA). Total antioxidant capacity (T-AOC) of three groups was detected by phenanthroline colorimetric method. The area under the ROC curve was used to analyze the diagnostic value of 8-OHdG, MDA, SOD and T-AOC in stroke. ResultsSerum 8-OHdG and MDA levels in the IS and ICH groups were higher than that of the normal control group (P<0.05). SOD and T-AOC levels in the IS and ICH groups were lower than that of the normal control group (P<0.05). The levels of serum 8-OHdG, SOD and T-AOC in the IS group were higher than those in the ICH group (P<0.05), while there was no significant difference between the two groups (P>0.05). 8-OHdG was positively correlated with MDA, SOD, T-AOC levels (P<0.01), MDA was not correlated with SOD (P>0.05), and MDA was positively correlated with T-AOC (P <0.05). The specificity of 8-OHdG, MDA, SOD and T-AOC in diagnosing IS and healthy subjects was 84%, 82%, 89% and 91%, respectively. The specificity of 8-OHdG, MDA, SOD and T-AOC in the diagnosis of ICH and healthy persons was 71%, 84%, 79% and 72%, respectively. 8-OHdG and T-AOC were closely related to stroke and protective factors for stroke. MDA and SOD were closely related to stroke and are risk factors for stroke. ConclusionSerum 8-OHdG, MDA, SOD and T-AOC are sensitive indicators of oxidative stress in stroke patients. The levels of 8-OHdG, SOD and T-AOC are significantly correlated with the severity of stroke, and have a high practical value for the differential diagnosis of stroke patients.