当归提取物抗电离辐射损伤在小鼠脾脏组织中的比较蛋白质组学研究

目的：探讨当归提取物对⁶⁰Coγ电离辐射损伤小鼠的脾脏组织蛋白质差异表达的影响及作用机制。方法：通过⁶⁰Coγ射线照射昆明种小鼠构建电离辐射损伤模型。运用iTRAQ联合LC-MS/MS技术对小鼠脾脏组织进行蛋白质组学检测,采用DAVID 6.8对差异蛋白进行Gene Ontology（GO）分析和KEGG Pathway显著富集性分析。结果：收集到240个差异蛋白参与的10条KEGG信号通路（P<0.05）,其中细胞色素B-245、髓过氧化物酶、主要组织相容性复合体、细胞表面糖蛋白TROP2等四个差异蛋白在吞噬体和抗原处理与呈递两条通路富集比较明显。结论：在小鼠脾脏组织中当归提取物抗电离辐射损伤的差异蛋白主要富集于吞噬体和抗原处理与呈递通路,当归提取物抗电离辐射损伤的机制可能与吞噬体和抗原处理与呈递参与的免疫调节相关。

Comparative proteomics study of angelica extracts on mouse spleen tissues damaged by ionizing radiation

OBJECTIVE To investigate the effect of angelica extracts on the differential expression of protein and possible mechanisms in spleen tissue of mice damaged ⁶⁰Coγ ionizing radiation. METHODS The ionizing radiation injury model was constructed by irradiating Kunming mice with ⁶⁰Coγ ray. Proteomics analysis of mouse spleen tissues was performed by iTRAQ combined with LC-MS/MS, and differential proteins were analyzed for significant enrichment by Gene Ontology (GO) and KEGG pathway using DAVID 6.8. RESULTS Ten KEGG pathways involved in 240 differentially expressed proteins were identified (P<0.05), among which cytochrome B-245, myeloperoxidase, major histocompatibility complex and cell surface glycoprotein TROP2 were significantly enriched in phagosome and antigen processing and presentation pathways. CONCLUSION The differentially expressed proteins of angelica extracts against ionizing radiation injury in mouse spleen tissues are mainly enriched in phagosome and antigen processing and presentation pathways, which may be related to the immune regulation involved in phagosomes and antigen processing and presentation.