| 新生大鼠少突胶质前体细胞的培养与鉴定 |
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| 引用本文： | 王丽雁,蔡文琴,陈鹏慧.新生大鼠少突胶质前体细胞的培养与鉴定[J].第三军医大学学报,2008,30(6):489-491. |
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| 作者姓名： | 王丽雁 蔡文琴 陈鹏慧 |
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| 作者单位： | 第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所,重庆,400038 |
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| 摘 要： | 目的 在体外培养条件下获取纯度较高的少突胶质前体细胞(oligodendrocyte progenitor cells,OPCs).方法 原代混合胶质培养,利用振荡法和差速贴壁法分离纯化少突胶质前体细胞,再用无血清化学条件培养基培养.免疫荧光法及膜片钳记录细胞电生理特性进行鉴定.结果 振荡分离后超过90%细胞为NG2阳性OPCs:OPCs电生理特性:输入阻抗Ra相对较高;电压依赖的K 电流具有外向整流特性;对河豚毒素(tetrodotoxin,TTX)敏感的小的内向Na 流,不能产生动作电位.结论 振荡法结合差速贴壁可以获得纯度较高的OPCs;OPCs具有区别于神经元、星型胶质细胞、小胶质细胞的独有电生理特性.
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| 关 键 词： | 少突胶质前体细胞 细胞培养 大鼠 |
| 文章编号： | 1000-5404(2008)06-0489-03 |
| 修稿时间： | 2007年10月29 |
Isolation and identification of oligodendrocyte progenitor cells from neonatal rats |
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| WANG Li-yan,CAI Wen-qin,CHEN Peng-hui.Isolation and identification of oligodendrocyte progenitor cells from neonatal rats[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(6):489-491. |
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| Authors: | WANG Li-yan CAI Wen-qin CHEN Peng-hui |
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| Institution: | Department of Neurobiology;College of Medicine;Third Military Medical University;Chongqing 400038;China |
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| Abstract: | Objective To develop new methods to cultivate, retrieve and purify oligodendrocyte progenitor cells (OPCs). Methods Primary OPCs were isolated from mixed glial cultures of newborn Sprague Dawley rat forebrains by using a selective detachment procedure, further purified by differential adhesion, and maintained in a chemical conditioned medium. The characteristic of OPCs was determined by both electrophysiological recording and immunostaining. Results Immunocytochemical analysis revealed that more than 90% of... |
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| Keywords: | oligodendrocyte progenitor cells cell cultures rat |
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