| 紫杉醇对人食管癌Eca109细胞株生长的影响 |
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| 引用本文： | 侯俊民,束永前,赵志泉.紫杉醇对人食管癌Eca109细胞株生长的影响[J].南京医科大学学报,2001,21(3):235-238. |
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| 作者姓名： | 侯俊民 束永前 赵志泉 |
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| 作者单位： | [1]南京医科大学第一附属医院消化研究室，江苏南京210029 [2]南京医科大学第一附属医院消化 |
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| 基金项目： | 江苏省教委自然科学基金资助项目 ( KJD3 2 0 0 0 5) |
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| 摘 要： | 目的：观察不同浓度的紫杉醇对食管癌细胞Eca109的影响。方法：应用四甲基偶氮唑蓝（MTT）实验，细胞形态学观察及流式细胞仪等方法对人食管癌细胞Eca109进行检测和观察。结果：MTT实验显示紫杉醇可抑制食管癌细胞增殖；光镜及电镜可发现药物作用组细胞核固缩、解聚以及凋亡小体，流式细胞仪检测显示G1峰前有明显的凋亡峰。细胞周期分析提示紫杉醇可将Eca细胞阻滞于G2－M期，且与浓度相关。结论：紫杉醇对人食管癌细胞系Eca109细胞的生长有明显的抑制作用，使细胞分裂阻滞于G2－M期，并诱导肿瘤细胞凋亡。
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| 关 键 词： | 紫杉醇 食管肿瘤 细胞周期 细胞凋亡 食管癌 |
| 文章编号： | 1007-4368(2001)03-0235-04 |
| 修稿时间： | 2000年11月22 |
The Effect of Paclitaxel on Esophageal Carcinoma Cell Line Eca109 in Vitro |
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| HOU Jun min,SHU Yong qian ,ZHAO Zhi quan.The Effect of Paclitaxel on Esophageal Carcinoma Cell Line Eca109 in Vitro[J].Acta Universitatis Medicinalis Nanjing,2001,21(3):235-238. |
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| Authors: | HOU Jun min SHU Yong qian ZHAO Zhi quan |
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| Institution: | HOU Jun min,SHU Yong qian 1,ZHAO Zhi quan |
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| Abstract: | Objective:To observe the effects of paclitaxel on esophageal carcinoma cell 109(Eca109) with different concentration and duration. Methods: With different concentration and duration of paclitaxel, the Eca109 cell activity, morphologic changes and cell DNA distribution were studied. Results:Paclitaxel could inhibit the proliferation of Eca109 cells in vitro, which was associated with arrest of G 2 M phase and with inducing apoptosis. Chromation condensation, nucleic fragmentation and apoptic body formation were found; folw cytometry analysis showed a sub G 1 cell peak. Conclusion:Paclitaxel can induce apoptosis and block cell cycle of esophageal cells. |
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| Keywords: | paclitaxel carcinoma esophageal cell cell cycle apoptosis |
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